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  Marine biopolymers as novel emulsifiers, foaming and gelling agents.


   School of Life Sciences

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Prof Tony Gutierrez  No more applications being accepted  Competition Funded PhD Project (European/UK Students Only)

About the Project

Proteins are an important class of functional molecules in a wide range of applications across the food, cosmetic and pharmaceutical industries. Applications include emulsification of oil, foaming and gelation. Many of the proteins used in these applications are of animal origin (egg and milk), although plant proteins such as soy are also used. The use of animal proteins, in particular, but also plant proteins, have been deemed unsustainable since they can be severely affected by climatic conditions, political upheaval etc. However, plant proteins often have inferior functionality to animal proteins. With a view to increase their competitiveness and deliver to consumer demand for natural and environmentally-friendly ingredients, a number of manufactures (e.g. Food & Drink, Cosmetics & Healthcare) are searching for novel, natural and eco-friendly SAs from sustainable and potentially underexploited sources, such as the marine environment.

This project will exploit the ability of marine bacteria to produce glycoproteins rich in uronic acids with demonstrated emulsifying properties. To date, however, the detailed properties of these glycoproteins, their structural features that give rise to the functional properties, and the interactions with other common food ingredients such as polysaccharide gums, has been largely unstudied. If these molecules are to be exploited as functional ingredients in industrial processes it is essential that this information is gathered and synthesised into a coherent theory to explain the structure-function relationship of the glycoproteins. This research will be nested in an environment of translational science that is both operating at the fundamental (bio)chemistry level, whilst also relevant to, and amenable for, exploitation by industry at the food and pharma levels. The research opens up the possibility of identifying glycoproteins with specific activities leading to commercial exploitation of a new generation of natural, sustainable and renewable functional food ingredients.

Ideally, though not compulsory, the student will have experience in microbiology and working with surface-active compounds/proteins (surfactants and/or emulsifiers), their production and extraction. The supervisory team comprises members with extensive experience and expertise in glycoprotein and protein surfactants and emulsifiers, and their production and physicochemical characterisation from microbial systems.

The project is suitable for a student with a strong microbiological and/or molecular background, ideally a first-class degree in Microbiology/Molecular Biology in marine sciences.

Informal enquiries to Dr. Stephen Euston ([Email Address Removed]). Interested applicants should send a CV, cover letter and provide contact details of two to three potential referees to Dr. Euston.

Funding Notes

This is a James-Watt PhD Scholarship that fully covers university student fees and stipend for up to 4 years.

References

1. M. Iljina, S.R. Euston, H.T. Bookey. (2013) Laser-induced volume changes during confocal Raman microscopy of whey-protein-stabilized emulsions and their relationship to protein content and particle diameters, Journal of Raman Microscopy. 44: 1084-1088.

2. S.R. Euston. (2013) Modelling and Computer Simulation of Food Structures, in Food Microstructures: Microscopy, Measurement and Modelling (V. Morris & K.Groves, Eds), Woodhead Publishing, London.

3. S.R.Euston,. (2014) Molecular Simulation of Adsorption of Hydrophobin HFBI to the Air-Water, DPPC-Water and Decane-Water Interfaces, Food Hydrocolloids. 42: 66-74.

4). Gutierrez, T., B. Mulloy, C. Bavington, K. Black, and D. H. Green. (2007) Partial purification and chemical characterization of a glycoprotein (putative hydrocolloid) emulsifier produced by a marine Antarctobacter species. Appl. Microbiol. Biotechnol. 76:1017-1026.

5). Gutierrez, T., B. Mulloy, K. Black, and D. H. Green. (2007) Glycoprotein emulsifiers from two marine Halomonas species: chemical and physical characterization. J. Appl. Microbiol. 103:1716-1727.

6). Gutierrez T, Morris, G., Green DH. (2009) Yield and physicochemical properties of EPS from Halomonas sp. strain TG39 identifies a role for protein and anionic residues (sulphate and phosphate) in emulsification of n-hexadecane. Biotechnol. Bioeng. 103: 207-216.

7). Gutierrez T, Shimmield T, Haidon C, Black K, Green DH. (2008) Emulsifying and metal ion binding activity of a glycoprotein exopolymer produced by Pseudoalteromonas sp. strain TG12. Appl Environ Microbiol 74:4867-4876.

8). L. Campbell, X. Gu, S.J. Dewar & S.R. Euston. (2009) Effects of different oils on the properties of soy protein isolate emulsions and gels, Food Research International, 42: 925-932.

9). S.R.Euston, A.A. Al-Bakkush & L. Campbell. (2009) Comparing the heat stability of soya protein and milk whey protein emulsions, Food Hydrocolloids, 23: 2485-2492.