Investigation of the replicative organelle of a positive-stranded RNA virus at University of Reading on FindAPhD.com

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Dr B Neuman Applications accepted all year round

About the Project

Positive-stranded RNA viruses replicate on intracellular membranes. What is particularly interesting is that these viruses cause the host cell to produce characteristic networks of lipid tubes and vesicles, and that similar structures are made by members of a virus family. Members of the coronavirus family create chaotic "convoluted membrane" structures and double-membraned vesicles (DMVs) from the endoplasmic reticulum of an infected cell.

We are investigating the formation and destruction of these structures, to better understand how they are related to the way the virus replicates and interacts with the cell. Investigations will include cryo-electron microscopy and tomography to examine the ultrastructure of the DMVs, use of modified viruses that are deficient in certain aspects of replication, reverse genetics and protein biochemistry.

Funding Notes

I am seeking capable, talented students who have obtained funding for a studentship. I am also willing to support you in making new applications for funding, but I do not have any unfilled, funded positions available in the lab at this time.

References

1. Serrano P et al., Nuclear magnetic resonance structure of the nucleic acid-binding domain of severe acute respiratory syndrome coronavirus nonstructural protein 3. J Virol. 2009 83(24):12998-3008.
2. Neuman BW et al., Proteomics analysis unravels the functional repertoire of coronavirus nonstructural protein 3. J Virol. 2008 82(11):5279-94.
3. Saunders AA et al., Mapping the landscape of the lymphocytic choriomeningitis virus stable signal peptide reveals novel functional domains. J Virol. 2007 81(11):5649-57
4. Neuman BW et al., Supramolecular architecture of severe acute respiratory syndrome coronavirus revealed by electron cryomicroscopy.
J Virol. 2006 80(16):7918-28
5. Neuman BW et al., Inhibition, escape, and attenuated growth of severe acute respiratory syndrome coronavirus treated with antisense morpholino oligomers.
J Virol. 2005 79(15):9665-76.

Where will I study?

University of Reading

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Investigation of the replicative organelle of a positive-stranded RNA virus

University of Reading School of Biological Sciences