Raman microspectroscopic analysis of live cells during abiotic perturbations

Live cell imaging is an important analytical tool for many areas of biomedical research including cell biology, neurobiology, pharmacology and developmental biology. With the continuing development of Raman microscopy this analytical technique now offers an alternative, label-free imaging approach capable of providing vast amounts of spatially resolved biochemical information.

The aim of this project will be to develop live cell Raman imaging using a Raman microscope coupled to a cell incubator in which cells can be grown and maintained in normal culturing conditions by regulating humidity, carbon dioxide levels and temperature. Once optimum conditions have been established the cell environment will be perturbed in a variety of ways including, the addition of specific chemicals and drugs to the medium, carbon dioxide levels, temperature and potentially mechanical forces, in order to monitor the biochemical response of the cells. As well as carrying out cell culture and Raman spectroscopy, the project will involve the development of data analysis techniques for the interpretation of spatially resolved biochemical information. This project is suitable for those with a background in chemistry, biochemistry or biology.

The recently established Department of Chemistry at Lancaster is developing into an outstanding environment for research and teaching. In the 2014 REF result, the Department was ranked 10th in the UK for research output quality, with 94% considered world leading and internationally significant. An investment of over £26 million from the University in new laboratories, instrumentation and equipment underpins the new development. We have also recently been awarded a significant European Regional Development Fund grant for the development of industry-based research based in the Department, to the value of £11 million. Research will be conducted in new, custom-designed research laboratories, with access to state-of-the-art equipment. See www.lancaster.ac.uk/chemistry for more details.

Applicants should hold, or expect to receive, a 1st class or good 2:1 Masters-level degree or equivalent in Chemistry.

Applications should be made via Lancaster University’s online application system (see http://www.lancaster.ac.uk/study/postgraduate/how-to-apply-for-postgraduate-study/ for details