About the Project
Understanding how proteins are sorted into different intracellular organelles is fundamental to our understanding of eukaryotic cells. Cells use this compartmentalization of processes to functionally separate reactions that would be incompatible yet this imposes a huge sorting problems. This project aims to gain real mechanistic insight into the import of proteins in one of these organelles the peroxisome. While many of the proteins that are involved in the process are known, the real fundamental mechanistic and structural insights are lacking as the precise order of interaction of proteins in the import mechanism is unclear. In this project you will study this process in detail so that we can start to really understand how simple protein-protein interactions can lead to the transport of large proteins across a biological membrane. In the project you will work with an established team of chemists and biologists to study this process by expressing proteins and chemical affinity probes to start to dissect individual interactions not just in solution but also on surfaces that more closely mimic the membrane environment where import occurs.
Funding Notes
BBSRC White Rose Mechanistic Biology DTP 4 year studentship.
Studentships covers UK/EU fees and stipend (c.£14,553) for 4 years to start in Oct 2018. Applicants should have/be expecting at least a 2.1 Hons. degree in a relevant subject. EU candidates require 3 years of UK residency in order to receive full studentship.
Not all projects advertised will be funded; the DTP will appoint a limited number of candidates via a competitive process and the projects selected by the successful candidates will be funded.
There are 2 stages to the application process. Please see our website for more information: http://www.fbs.leeds.ac.uk/postgraduate/phdopportunities.php
References
Skoulding NS, Chowdhary G, Deus MJ, Baker A, Reumann S, Warriner SL (2015) Experimental validation of plant peroxisomal targeting prediction algorithms by systematic comparison of in vivo import efficiency and in vitro PTS1 binding affinity. Journal of Molecular Biology, 427, pp 1089-8638.
Lanyon-Hogg T, Hooper J, Gunn S, Warriner SL, Baker A (2014) PEX14 binding to Arabidopsis PEX5 has differential effects on PTS1 and PTS2 cargo occupancy of the receptor. FEBS Lett, 588, pp 2223-2229
Paudyal R, Jamaluddin A, Warren JP, Doyle SM, Robert S, Warriner SL, Baker A. (2014) Trafficking modulator TENin1 inhibits endocytosis, causes endomembrane protein accumulation at the pre-vacuolar compartment and impairs gravitropic response in Arabidopsis thaliana. Biochem J, 460, pp 177-185
Brown L-A, Paudyal R, Baker A, Larson TR, Graham IA, Hawes C, Warriner SL (2013) An inhibitor of oil body mobilization in Arabidopsis, New Phytol, 200, pp 641-649
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