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| Multiphoton Fluorescence Lifetime Imaging | ||||||||||||
Multiphoton microscopy is a high resolution 3D optical imaging modality that has seen widespread use for microscopy at a depth of <1 mm within biological samples. In combination with fluorescence lifetime imaging (FLIM) techniques, multiphoton microscopy enables quantitative high resolution imaging of protein-protein interactions by FRET and environmental sensing in live cells. The aim of the project is to develop multiphoton FLIM for use in thick biological samples by application of adaptive optics. The student will integrate a membrane deformable mirror into the excitation light path of the microscope and develop a sensing methodology to enable active feedback of aberrations within the sample. Correction of system and sample induced aberrations will be validated using tissue phantoms and specially prepared biological samples. Funding Notes Award type: Funded by the (£8M) Comprehensive Cancer Imaging Centre of UCL and KCL, though a joint Cancer Research UK and EPSRC initiative. Fees paid at UK/EU rate. Stipend at £14,988pa Selection process All shortlisted applicants will be interviewed by at least two academics from the Division. For more information and details on how to apply, please visit www.kcl.ac.uk/schools/health/study/studentships/ More information: richard.hocking@kcl.ac.uk |
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