Protein-protein interactions in the pantothenate biosynthetic pathway

Protein-protein interactions are critical to the function of many signaling pathways. Their importance in the regulation of central metabolism is less well studied, however PPIs can serve to modulate the catalytic activity of enzymes. In this project you will investigate the structural basis for interactions with enzymes of the pantothenate biosynthetic pathway previously identified via high-throughput screening. You will use a combination of approaches including fluorescence, calorimetry, X-ray crystallography and small angle X-ray scattering to define the shape and affinity of the complexes in solution and then assay the biochemical consequences of their formation.