About the Project
This project aims to identify candidate genes responsible for TuYV resistance in a recently mapped resistant brassica plant line using NGS technology. Candidate genes will be taken forward for proof of concept studies in a model plant system in this project.
A second objective is to analyse TuYV infection in pea (Pisum sativum). Recently, TuYV was detected in agronomically important pea crops. The extent and impact of the TuYV outbreaks in pea are still unknown and there is an urgent need to assess the risks TuYV presents for pea crops. The UK is the largest producer of frozen peas in Europe and annual production of more than 160,000 tonnes underpins the commercial importance of this crop. The aims of this component of the project is to identify the range of TuYV isolates capable of infecting pea, quantify virus titres in different commercial pea varieties during infection and determine the impact on growth and yield.
Techniques that will be undertaken during the project:
- Virus detection and quantification via enzyme-linked immunosorbent assay (ELISA) and RT-PCR
- Data analysis DNA-seq and phylogenetic analysis
- Molecular biological techniques like PCR, cloning, sequencing etc.
- Plant transformation
Based on your current searches we recommend the following search filters.
Based on your current search criteria we thought you might be interested in these.