This project is available to UK/EU candidates. Funding covers fees (UK/EU rate) and stipend for four years. Overseas candidates can apply providing they can pay the difference in fees and are from an eligible country. Candidates will be required to split their time between Manchester and Singapore, as outlined on View Website.
As an equal opportunities institution we welcome applicants from all sections of the community regardless of gender, ethnicity, disability, sexual orientation and transgender status. All appointments are made on merit.
Examples of recent related research from Manchester and A*STAR:
1] A vitamin K-dependent carboxylase is involved in antibiotic biosynthesis. B. J. C. Law, Y. Zhuo, D. Francis, M. Winn, Y. Zhang, M. Samborskyy, A. Murphy, P. F. Leadlay & J. Micklefield. Nature Catalysis 2018, 1, 977-984 (http://dx.doi.org10.1038/s41929-018-0178-2)
2] De novo Biosynthesis of 'Non-Natural' Thaxtomin Phytotoxins. M. Winn, D. Francis & J. Micklefield, Angew. Chem. Int. Ed. 2018, 57, 6830-6833. (http://dx.doi.org/10.1002/anie.201801525)
3] RadH: A Versatile Halogenase for Integration into Synthetic Pathways. B. R. K. Menon, E. Brandenburger, H. H. Sharif, U. Klemstein, M. F. Greaney & J. Micklefield Angew. Chem. Int. Ed. 2017, 56, 11841–11845 (http://dx.doi.org/10.1002/anie.201706342)
4] CRISPR –Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters. M. M. Zhang, F. Wong, Y. Wang, S. Luo, Y. H. Lim, E. Heng, W. L. Yeo, R. E Cobb, B. Enghiad, E.L. Ang & H. Zhao. Nature Chemical Biology 2017, 13, 607–609. (http://dx.doi.org/10.1038/nchembio.2341)
5] Twin-primer non-enzymatic DNA assembly: an efficient and accurate multi-part DNA assembly method. J. Liang, Z. Liu, X.Z. Low, E.L. Ang & H. Zhao. Nucleic Acids Research 2017, 45, e94 (https://doi.org/10.1093/nar/gkx132)
6] A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways. S. Shi, Y. Liang, M.M. Zhang, E.L. Ang & H. Zhao. Metabolic Engineering 2016, 33, 19-27 (https://doi.org/ 10.1016/j.ymben.2015.10.011).