Are you interested in a PhD in translational medicine? We have an exciting, fully-funded, PhD position to join our team to study novel RNA binding proteins in the area of cardio-renal disease. Cardiovascular Disease together with Chronic Kidney Disease are leading causes of death worldwide and critically remain without cures. RNA binding proteins control every aspect of the life cycle of RNA from translation to degradation. They also interact with non-coding RNAs such as microRNAs and long non-coding RNA. Over the last few years new and exciting technologies have been developed allowing us to study RNA binding proteins for the first time.
This project is at the forefront of research into cardio-renal diseases and has the potential to discover new proteins involved in disease, some of which may carry therapeutic potential. Via this project the selected PhD student will carry out multi-disciplinary translational research to identify protein partners of a newly identified RNA binding protein by employing affinity capture immunoprecipitation followed by mass-spectrometry (IP-MS). To reveal which of the newly identified proteins are differentially expressed the student will perform staining in tissues from disease models (in vivo). Genetic engineering will be applied to alter the expression of genes of interest using cutting-edge CRISP/cas and siRNA technologies. Inflammation, proliferation and apoptosis will be assessed in human cells cultured in 3 dimensions (3D organoids). The student will gain a vast array of techniques including genetic modulation of cells and many in vitro and in vivo methods.
BENEFITS: This position offers the possibility for travel to our partner laboratories (EMBL in Heidelberg and MIT Harvard University). The host university is an internationally recognised centre of research excellence and offers access to state of the art facilities to deliver high impact research. The University of Sheffield is in the top 100 best non-for-profit organisations in the world and has been voted the number 1 Russel University for student satisfaction in the UK.
The Medical School has a successful mentoring scheme for PhD students, which provides personal and professional development, in addition to a number of career and personal development courses. We offer bespoke training in key skills sought after in early career researchers, as well as opportunities to broaden your horizons in a range of non-academic sectors. We are proud holders of an Athena Swan Silver Award in recognition of our efforts to build a happy and rewarding environment for both female and male staff and students. Being supported by a UKRI grant means you can access additional funding for international research placements.
This work will be carried out the in the laboratory of Dr Maria Fragiadaki, for more information please visit our website: (https://www.sheffield.ac.uk/medicine/people/iicd/maria-fragiadaki
Candidates must have a first or upper second-class honors degree or significant research experience.
Interested candidates should in the first instance contact (Dr Maria Fragiadaki at [Email Address Removed]).
How to apply:
Please complete a University Postgraduate Research Application form available here: www.shef.ac.uk/postgraduate/research/apply
Please clearly state the prospective main supervisor in the respective box and select Infection, Immunity and Cardiovascular Disease as the department.
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