FindA University Ltd Featured PhD Programmes
Health Data Research UK Featured PhD Programmes
Peter MacCallum Cancer Centre Featured PhD Programmes

A whole genome CRISPRi mitophagy screen for regulators of mitochondrial protein import quality control


School of Biochemistry

Monday, December 07, 2020 Competition Funded PhD Project (UK Students Only)

About the Project

Mitophagy is a highly selective form of autophagy (cellular self‐eating) for the removal of damaged or redundant mitochondria via lysosomal degradation. It is an essential facet of the cellular response to physiological and environmental stress, and failures in mitophagy regulation are implicated in numerous human diseases (including neurodegenerative diseases), and also contribute to the ageing process. This project will use CRISPRi (CRISPR interference) to screen for proteins and pathways that coordinate mitophagy during mitochondrial stress. Mitophagy reporter cell‐lines will be treated with mitochondrial protein import blockers, and cells with impaired or elevated mitophagy responses will be isolated and target genes then identified. Training will be provided in e.g.: widefield and confocal microscopy, including automated high content analysis; CRISPRi assay design; bioinformatics and large data set analytical methods; mechanistic cell biology.

For further information including recent lab publications, visit: https://thelanelab.blogs.bristol.ac.uk

Twitter: @Jon_D_Lane

Funding Notes

This project is available under the SWBio DTP programme: Link to SWBio DTP website: View Website

DEADLINE FOR APPLICATIONS: Midnight on Monday 7 December 2020

How to apply: View Website

Information on eligibility: View Website

Email Now

Insert previous message below for editing? 
You haven’t included a message. Providing a specific message means universities will take your enquiry more seriously and helps them provide the information you need.
Why not add a message here

The information you submit to University of Bristol will only be used by them or their data partners to deal with your enquiry, according to their privacy notice. For more information on how we use and store your data, please read our privacy statement.

* required field

Your enquiry has been emailed successfully





FindAPhD. Copyright 2005-2020
All rights reserved.