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A whole genome CRISPRi mitophagy screen for regulators of mitochondrial protein import quality control

School of Biochemistry

Bristol United Kingdom Biochemistry Cell Biology

About the Project

Mitophagy is a highly selective form of autophagy (cellular self‐eating) for the removal of damaged or redundant mitochondria via lysosomal degradation. It is an essential facet of the cellular response to physiological and environmental stress, and failures in mitophagy regulation are implicated in numerous human diseases (including neurodegenerative diseases), and also contribute to the ageing process. This project will use CRISPRi (CRISPR interference) to screen for proteins and pathways that coordinate mitophagy during mitochondrial stress. Mitophagy reporter cell‐lines will be treated with mitochondrial protein import blockers, and cells with impaired or elevated mitophagy responses will be isolated and target genes then identified. Training will be provided in e.g.: widefield and confocal microscopy, including automated high content analysis; CRISPRi assay design; bioinformatics and large data set analytical methods; mechanistic cell biology.

For further information including recent lab publications, visit:

Twitter: @Jon_D_Lane

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