University of Cambridge Featured PhD Programmes
Imperial College London Featured PhD Programmes
University of Southampton Featured PhD Programmes
University of Dundee Featured PhD Programmes
The University of Manchester Featured PhD Programmes

ADAR2 RNA editing in CNS

  • Full or part time
  • Application Deadline
    Thursday, February 20, 2020
  • Funded PhD Project (Students Worldwide)
    Funded PhD Project (Students Worldwide)

Project Description

The main part of the work will use Drosophila but the student will also have access to Adar2 mutant mice which model the severe epilepsy caused by ADAR2 mutations in humans. Adar2 mutant mouse pups develop seizures and die at or before weaning.
In Drosophila we found that Adar mutant phenotypes can be rescued by increased autophagy which clears synaptic defects. The new student will join Anzer Khan who is near the end of his PhD to continue studying the rescues of Adar mutant phenotypes by Hsc70-4 to understand how Adar mutant defects arise and are rescued by increased Hsc70-4.
The Adar mutant defects may be due to loss of RNA editing in specific transcripts such as synaptotagmin, synapsin and cacophony that encode key synaptic proteins. We will try to rescue Adar mutant defects by expressing edited forms of cDNAs for these specific transcripts that are available within the Drosophila community

Keywords: RNA modification, ADAR, genetics

HOW TO APPLY: Register for this call using the registration form at http://ls-phd.ceitec.cz/http-ls-phd-ceitec-cz/ and submit required documents to receive support with the preparation of the application and all formalities. Your application package will be forwarded to the supervisor for preliminary revision.
Applicants who wish to pursue a degree at the CEITEC PhD program must hold the equivalent of a Master’s degree (MSc) in similar field (four- or five-year undergraduate degree). The application can be submitted before obtaining the Master’s degree, however, the applicant should obtain the degree within five months after the application deadline.

Funding Notes

Admission for studies, student registration for the full-time study program and proper fulfilment of student duties constitute the student’s right to a regular income of 22 000 CZK (850 EUR). Information is available at View Website

Living costs and other practicalities available at View Website

References

1. ADAR RNA editing in innate immune response phasing, in circadian clocks and in sleep. Sinigaglia K, Wiatrek D, Khan A, Michalik D, Sambrani N, Sedmík J, Vukić D, O'Connell MA, Keegan LP. Biochim Biophys Acta Gene Regul Mech. 2019 Mar;1862(3):356-369. Review. PMID:30391332
2. ADAR RNA editing in human disease; more to it than meets the I. Gallo A, Vukic D, Michalík D, O'Connell MA, Keegan LP. Hum Genet. 2017 Sep;136(9):1265-1278. Review. PMID: 28913566
3. The ADAR RNA editing enzyme controls neuronal excitability in Drosophila melanogaster. Li X, Overton IM, Baines RA, Keegan LP, O'Connell MA. Nucleic Acids Res. 2014 Jan;42(2):1139-51. doi: 10.1093/nar/gkt909. Epub 2013 Oct 16. PMID: 24137011
4. Functional conservation in human and Drosophila of Metazoan ADAR2 involved in RNA editing: loss of ADAR1 in insects. Keegan LP, McGurk L, Palavicini JP, Brindle J, Paro S, Li X, Rosenthal JJ, O'Connell MA. Nucleic Acids Res. 2011 Sep 1;39(16):7249-62. doi: 10.1093/nar/gkr423. Epub 2011 May 27. PMID: 21622951
5. Tuning of RNA editing by ADAR is required in Drosophila. Keegan LP, Brindle J, Gallo A, Leroy A, Reenan RA, O'Connell MA. EMBO J. 2005 Jun 15;24(12):2183-93. Epub 2005 May 26. PMID: 15920480

Email Now

Insert previous message below for editing? 
You haven’t included a message. Providing a specific message means universities will take your enquiry more seriously and helps them provide the information you need.
Why not add a message here
* required field
Send a copy to me for my own records.

Your enquiry has been emailed successfully





FindAPhD. Copyright 2005-2019
All rights reserved.