About the Project
In this project, we will determine the functional consequences of activating and inactivating small GTPases of the Arf and Rab families on polarized growth and development. In yeast, we will take advantage of two established light inducible targeting systems to regulate GTPase activity locally through GEFs and GAPs. We will establish GTPase-regulator circuits important for polarized growth in yeast. This knowledge, we will transfer into another highly polarized cell: the C. elegans zygote. We will determine the effect of functionally interfering with the homologous GTPase-regulator circuits on polarity establishment and maintenance from the zygote to the 4-cell stage using RNAi and CRISPR-Cas9 methodology. Finally, we will extend our findings to C. elegans intestinal epithelial cells.
Optogenetics, live cell imaging, molecular biology and biochemical techniques, CRISPR-Cas9, standard yeast and C. elegans techniques.
For more details please visit: https://polarnet-itn.eu/
As part of the project, the successful applicant will visit collaborating labs with in the PolarNet network to learn new techniques.
Ritz AM, Trautwein M, Grassinger F, Spang A. The prion-like domain in the exomer-dependent cargo Pin2 serves as a trans-Golgi retention motif. Cell Rep. 2014 Apr 10;7(1):249–60.
Poteryaev D, Datta S, Ackema K, Zerial M, Spang A. Identification of the switch in early-to-late endosome transition.
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