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(BBSRC DTP) Evolution in silico and in vitro: environmental change, mutation and space


Project Description

Understanding evolutionary processes requires an interplay between wet-lab experiment and computational simulation [1]. This project will use model systems for both to explore the interplay of evolutionary processes (primarily mutation) and the spatial environment.

REvoSim is a custom-written piece of software, developed by members of the supervisory team . It is capable of simulating evolution for large populations (>1 million) of individuals over long time periods (>5 million iterations). REvoSim uses a simplified model for computational efficiency, but incorporates many aspects of real biological evolution. It is a highly simplified system in which 64-bit digital organisms are modelled within a 1D or 2D RGB-colour based environment. Fitness depends on this environment. The organisms: have a coding and non-coding genome; can breed sexually or asexually; can move on breeding in 2D simulations; can mutate; can form different species; and have a limited or unlimited lifespan. The environment can be static or dynamic, and if the latter, can have variable rates of change. The system has a large number of variables, allowing the impact of each to be assessed; for example, the impact of rate of mutation on fitness, and the effect rate of environmental change has on the evolutionary patterns observed.

However, computational simulation results require truthing in wet-lab experiments. The organisms simulated in REvoSim bear similarity to microbes, which can also be evolved experimentally [1]. We have used such approaches to look at basic mechanisms of evolution, in particular the relationship of mutation and the environment [2], including the social environment [3]. This project will groundtruth the results of EvoSim simulations through complementary wet-lab work. Like REvoSim digital organisms, these can be studied over realistic time spans, and in large populations, both in spatial environments (agar plates) [4] and non-spatial environments (shaken broth). This project will use the bacterium Escherichia coli and yeast Saccharomyces cerevisiae to test qualitative and quantitative predictions from EvoSim.

This interdisciplinary combination of in-silico and lab-based study of evolution, can provide new insights into how evolution works. The consilience between complementary approaches will hone in on the driving forces behind different evolutionary patterns. This represents a unique opportunity for applicants interested in both computational biology approaches and labwork, and will allow the student to both write software in C++ and train in wet-lab techniques: a combination which will lend itself to multiple future career paths.

Entry Requirements:
Applications are invited from UK/EU nationals only. Applicants must have obtained, or be about to obtain, at least an upper second class honours degree (or equivalent) in a relevant subject.

Funding Notes

This project is to be funded under the BBSRC Doctoral Training Programme. If you are interested in this project, please make direct contact with the Principal Supervisor to arrange to discuss the project further as soon as possible. You MUST also submit an online application form - full details on how to apply can be found on the BBSRC DTP website View Website

As an equal opportunities institution we welcome applicants from all sections of the community regardless of gender, ethnicity, disability, sexual orientation and transgender status. All appointments are made on merit.

References

[1] T. Hindre, et al. New insights into bacterial adaptation through in vivo and in silico experimental evolution. Nat. Rev. Microbiol. 10, 352-365 (2012).
[2] Krašovec, R. et al.Spontaneous Mutation Rate Is a Plastic Trait Associated with Population Density across Domains of Life. PLoS Biology, 15, e2002731. (2017)
[3] Krašovec, R. et al. (2014) Mutation rate plasticity in rifampicin resistance depends on Escherichia coli cell–cell interactions. Nat Commun, 5, 3742.
[4] O. Hallatschek, et al. Genetic drift at expanding frontiers promotes gene segregation. Proc. Natl. Acad. Sci. U. S. A. 104, 19926-19930 (2007).

How good is research at University of Manchester in Earth Systems and Environmental Sciences?

FTE Category A staff submitted: 42.13

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