About the Project
The student will then apply novel bioanalytical tools to these models to probe the effects of each of the mutations, alone and in combination. Specifically the student will use a combination of mass spectrometry and vibrational spectroscopy approaches that are being pioneered in Manchester for biomolecular characterization. Signatures generated using these techniques will then be validated using RNAseq.
Taken together these studies represent a novel approach and make use of an existing collaboration to integrate advanced cell culture modelling with a unique analytical strategy. Results from these studies will provide important insights into the effect of these driver mutations upon cellular physiology which will pave the way for development of novel screening and detection methodologies.
The student will develop a wide range of transferable lab and analytical skills to enhance their career development.
Applications are invited from UK/EU nationals only. Applicants must have obtained, or be about to obtain, at least an upper second class honours degree (or equivalent) in a relevant subject.
2. Denbigh, J. L., Perez-Guaita, D., Vernooij, R., Tobin, M., Bambery, K., Xu, Y., Southam, A., Khanim, F., Drayson, M., Lockyer, N., Goodacre, R., and Wood, B. Probing the action of a novel anti-leukaemic drug therapy at the single cell level using modern vibrational spectroscopy techniques, Scientific Reports, 2017. 7(1): 2649
3. McCormick, A., Edmondson RJ et al., Ovarian Cancers Harbour Defects in Non-Homologous End Joining Resulting in Resistance to Rucaparib. Clin Cancer Res, 2016
4. A. L. M. Batista de Carvalho, M. Pilling, P. Gardner, J. Doherty, G. Cinque, K. Wehbe, C. Kelley, L. A. E. Batista de Carvalho and M. P. M. Marquesa, Chemotherapeutic Response to Cisplatin-like Drugs in Human Breast Cancer Cells Probed by Vibrational Microspectroscopy. Faraday Discussion, 2016, 187, 273-298
5. M. Pilling and P. Gardner, Fundamental developments in infrared spectroscopic imaging for biomedical applications. Chemical Society Reviews, 2016, 45, 1935 - 1957
Based on your current searches we recommend the following search filters.
Based on your current search criteria we thought you might be interested in these.