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(BBSRC DTP) Sharpening up protein NMR: ultra-high resolution experiments


Project Description

The project directly addresses the topic of technology development (i.e. new NMR experiments), as well as providing advanced training in the skills required to operate the latest NMR spectrometers at an expert level and to apply computational techniques to biological data. As more and more users are limited to using NMR ready-prepared pulse programs, the skills of de novo pulse programming for biological applications are becoming scarce in the UK. One aim of this project is to fill this training gap.

NMR is one of the most important techniques for gaining information on proteins in solution, but the ability to resolve individual NMR signals is a prerequisite for successful analysis. The new class of pure shift NMR experiments has been shown to greatly enhance resolution for small molecules in solution, and initial results on proteins are very promising. Here we are going to develop a range of such methods for the study of protein structure and dynamics. Pure shift methods1-3 rely on suppressing the multiplet structure caused by spin-spin interactions to improve resolution, and are particularly promising for the very important class of proteins that have dynamic, flexible functional segments with high local motion.4,5 This project will deliver a family of pure shift methods which will enable much more detailed study of such proteins.

The project will be supervised by a team of researchers from the School of Chemistry at the University of Manchester, world-leading experts in the development of NMR spectroscopy methods and their application to biomolecules. The University of Manchester has excellent facilities for this research, with over 15 high resolution NMR spectrometers ranging from 300 to 800 MHz. This interdisciplinary project spans a range of disciplines from physics to biology and will provide extensive training in a range of research methods, allowing the student to acquire a broad set of skills including practical NMR spectroscopy, spin physics, biotechnology, protein expression, and scientific programming.

Contact for further information:

http://www.manchester.ac.uk/research/A.Golovanov/
https://www.research.manchester.ac.uk/portal/Gareth.Morris.html
https://www.research.manchester.ac.uk/portal/mathias.nilsson.html
http://nmr.chemistry.manchester.ac.uk/

Entry Requirements:
Applications are invited from UK/EU nationals only. Applicants must have obtained, or be about to obtain, at least an upper second class honours degree (or equivalent) in a relevant subject.


Funding Notes

This project is to be funded under the BBSRC Doctoral Training Programme. If you are interested in this project, please make direct contact with the Principal Supervisor to arrange to discuss the project further as soon as possible. You MUST also submit an online application form - full details on how to apply can be found on the BBSRC DTP website View Website

As an equal opportunities institution we welcome applicants from all sections of the community regardless of gender, ethnicity, disability, sexual orientation and transgender status. All appointments are made on merit.

References

1. L. Paudel, R. W. Adams, P. Kiraly, et al., Angew. Chem. Int. Ed., 2013, 52, 11616.
2. P. Kiraly, R. W. Adams, L. Paudel, et al., J. Biomol. NMR, 2015, 62, 43.
3. M. Foroozandeh, R. W. Adams, N. J. Meharry, et al., Angew. Chem. Int. Ed., 2014, 53, 6990.
4. E. Carmelo, D. Barilla, A. P. Golovanov, et al., J. Biol. Chem., 2005, 280, 28683.
5. A. P. Golovanov, G. M. Hautbergue, A. M. Tintaru, et al., RNA, 2006, 12, 1933.

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