NMR is one of the most important techniques for gaining information on proteins in solution, but the ability to resolve individual NMR signals is a prerequisite for successful analysis. The new class of pure shift NMR experiments has been shown to greatly enhance resolution for small molecules in solution, and initial results on proteins are very promising. Here we are going to develop a range of such methods for the study of protein structure and dynamics. Pure shift methods1-3 rely on suppressing the multiplet structure caused by spin-spin interactions to improve resolution, and are particularly promising for the very important class of proteins that have dynamic, flexible functional segments with high local motion.4,5 This project will deliver a family of pure shift methods which will enable much more detailed study of such proteins.
The project will be supervised by a team of researchers from the School of Chemistry at the University of Manchester, world-leading experts in the development of NMR spectroscopy methods and their application to biomolecules. The University of Manchester has excellent facilities for this research, with over 15 high resolution NMR spectrometers ranging from 300 to 800 MHz. This interdisciplinary project spans a range of disciplines from physics to biology and will provide extensive training in a range of research methods, allowing the student to acquire a broad set of skills including practical NMR spectroscopy, spin physics, biotechnology, protein expression, and scientific programming.
Applications are invited from UK/EU nationals only. Applicants must have obtained, or be about to obtain, at least an upper second class honours degree (or equivalent) in a relevant subject.
Contact for further Information
For more details contact Dr Mathias Nilsson ([Email Address Removed])
This project is to be funded under the BBSRC Doctoral Training Programme. If you are interested in this project, please make direct contact with the Principal Supervisor to arrange to discuss the project further as soon as possible. You MUST also submit an online application form - full details on how to apply can be found on the BBSRC DTP website www.manchester.ac.uk/bbsrcdtpstudentships
1. L. Paudel, R. W. Adams, P. Kiraly, et al., Angew. Chem. Int. Ed., 2013, 52, 11616.
2. P. Kiraly, R. W. Adams, L. Paudel, et al., J. Biomol. NMR, 2015, 62, 43.
3. M. Foroozandeh, R. W. Adams, N. J. Meharry, et al., Angew. Chem. Int. Ed., 2014, 53, 6990.
4. E. Carmelo, D. Barilla, A. P. Golovanov, et al., J. Biol. Chem., 2005, 280, 28683.
5. A. P. Golovanov, G. M. Hautbergue, A. M. Tintaru, et al., RNA, 2006, 12, 1933.