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Biomarkers of Genotoxic and Reprotoxic Effects after chemical exposure in vitro in human cells

   Faculty of Life Sciences

About the Project

In human monitoring, procedure measurement obtained from endpoints from endpoints to determine genetic damage are sometimes referred to as biomarkers. Depending on their nature, they may be biomarkers of exposure, effect, or susceptibility. A causal relationship has been proposed for cytogenetic damage in peripheral lymphocytes and subsequent cancer risk.

Biomarkers include measurement of chromosome damage using fluorescence in situ hybridisation (FISH) techniques, altered level in serum of Ras p21 oncoproteins or p53 tumour suppressor genes, and DNA strand breakage in the single cell electrophoresis assay (Comet assay). Damage can be measured in individuals of different metabolic genotypes or different disease states, such as respiratory diseases (lung cancer patients, chronic obstructive pulmonary diseases and asthma, head, and neck cancer), diabetes, tuberculosis, etc. As well as for the examination of somatic cells (human lymphocytes in blood), the Comet assay can also be used effectively in human sperm to give some measure of DNA integrity in future generation. The effect of an individual's genetic constitution and lifestyle e.g., diet, smoking and drinking habits can also affect responses.

The proposed studies would examine the effects of various compounds, after in vitro exposure, in ex vivo / in vitro patients primarily in human blood, but also in sperm, to determine the contribution of the various biomarkers of different disease states. Responses would be compared with effects in healthy individuals as well as the confounding effects of individual`s genetic constitution and lifestyle. In the first instance the disease of various cancer would be investigated, but other disease states could be considered.

The appointed candidate will join our School of Chemistry and Biosciences at the University of Bradford.

Funding Notes

This is a self-funded PhD project; applicants will be expected to pay their own fees or have a suitable source of third-party funding. A bench fee also applies in addition to tuition fees.

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