Linolenic acid (GLA, ω-6 family) is a medicinally and nutritionally important polyunsaturated fatty acid (PUFA) with a broad spectrum of applications, extending from food supplements to anti-cancer formulations. Several fungal species are capable of accumulating high amounts of intracellular lipids of rarely occurring composition. Strains of Mortierella sp. and produce lipids enriched in GLA that can be used in food products owing to their GRAS (Generally Regarded as Safe) status. This project involves the screening of several oleaginous fungal strains and further investigation on the most promising candidates on PUFA production. Fungal culitvations will be carried out in conditions that will promote high accumulation of microbial lipids. Moreover, supercritical CO2 technology will be applied for the extraction and purification of microbial lipids and potential food applications will be assessed.