University of Edinburgh Featured PhD Programmes
Health Data Research UK Featured PhD Programmes
University College London Featured PhD Programmes

Budding yeast as a model for sudden cardiac death


Biosciences

This project is no longer listed on FindAPhD.com and may not be available.

Click here to search FindAPhD.com for PhD studentship opportunities
Dr Thomas Hoefken Applications accepted all year round Self-Funded PhD Students Only

About the Project

Sudden unexpected cardiac death is responsible for about half of all heart disease deaths. It is an unexpected death caused by a change in heart rhythm. The underlying mechanisms are still not fully understood. We have recently identified several point mutations in the gene PPA2 in infants and young people that died from sudden cardiac arrest. PPA2 encodes a mitochondrial enzyme and the identified mutations lead to mitochondrial defects. However, the exact molecular mechanisms remain unclear. Much of our understanding of mitochondrial function and defects is based on studies in the budding yeast Saccharomyces cerevisiae. Since PPA2 is highly conserved from yeast to men, budding yeast is an ideal system for the analysis of the effects of PPA2 mutations found in patients. It will be tested whether these point mutations have an effect on the enzyme activity and protein stability of Ppa2. It has been suggested that Ppa2 functions as a homodimer. It will therefore be tested whether Ppa2 forms dimers and whether the identified mutations have an effect on protein dimerization. It will also be tested whether Ppa2 mutations affect mitochondrial function and morphology.
A wide range of methods will be employed. This will include cloning techniques, co-immunoprecipitation, SDS-PAGE, immunoblotting, enzyme activity assays, fluorescence microscopy and yeast techniques.

Funding Notes

Brunel offers a number of funding options to research students that help cover the cost of their tuition fees, contribute to living expenses or both. See more information here: https://www.brunel.ac.uk/research/Research-degrees/Research-degree-funding
Recently the UK Government made available the Doctoral Student Loans of up to £25,000 for UK and EU students and there is some funding available through the Research Councils. Many of our international students benefit from funding provided by their governments or employers. Brunel alumni enjoy tuition fee discounts of 15%.

References

Kennedy H, et al. (2016). Sudden cardiac death due to deficiency of the mitochondrial inorganic pyrophosphatase PPA2. Am. J. Hum. Genet. 99:674-82
Guimier A, et al. (2016). Biallelic PPA2 mutations cause sudden unexpected cardiac arrest in infancy. Am. J. Hum. Genet. 99:666-73
Search Suggestions

Search Suggestions

Based on your current searches we recommend the following search filters.



FindAPhD. Copyright 2005-2020
All rights reserved.