About the Project
The ‘N-degron pathway’ is a specific pathway for protein degradation that targets proteins for destruction based on their N-terminal amino acid residue (see Gibbs et al. 2014 Trends in Cell Biology). It was previously shown that regulation of transcription factor stability via this pathway is important for sensing low oxygen stress (hypoxia), a situation that frequently occurs during floods, and nitric oxide (NO), a gaseous molecule that regulates developmental transitions and environmental responses (Gibbs et al. 2011 Nature; Gibbs et al. 2014 Molecular Cell). More recently, we have linked this pathway to the regulation of a conserved protein complex that controls longer-term epigenetic responses to the environment by modifying histones in target genes (Gibbs et al. 2018 Nature Communications), and we are also investigating a new ‘branch’ of the system based on N-terminal acetylation of proteins (Gibbs et al. 2015 Trends in Plant Science). Despite its emergence as a major regulator of plant signal transduction, our knowledge of the range of protein targets and cellular processes regulated by the N-degron pathway in plants is currently limited. There are three key areas we are seeking to investigate wiht this project:
(i) Investigating the role of protein degradation via the N-degron pathway in controlling the epigenome, and its subsequent effects on development and environmental responsiveness.
(ii) Investigating links between N-terminal acetylation and protein stability in plants, by functionally characterising the enzymes involved, identifying protein targets, and linking the pathway to plant growth and stress-responses.
(iii) Investigating how components of the N-degron pathway regulate co-translational quality control to ensure the efficient production of proteins to maintain cellular function.
The majority of our work is carried out in the genetic model plant Arabidopsis, with the eventual aim of this research being to translate key findings into crop species, such as barley or rice. The research will be largely molecular based and will also include ‘omics’ approaches (such as ChIP-Seq and proteomics). The PhD candidate will therefore gain expertise in a wide range of cutting edge and transferable techniques.
Gibbs DJ, Isa NM, Movahedi M, Lozano-Juste J, Mendiondo GM, Berckhan S, Marín-de la Rosa N, Vicente Conde J, Sousa Correia C, Pearce, SP, Bassel GW, Hamali B, Talloji P, Tomé DFA, Coego A, Beynon J, Alabadí D, Bachmair A, León J, Gray JE, Theodoulou FL, Holdsworth MJ (2014) Nitric oxide sensing in plants is mediated by proteolytic control of Group VII ERF transcription factors. Molecular Cell. 53(3): 369-379
Gibbs DJ, Tedds HM, Labandera A-M, Bailey M, White MD, Hartman S, Sprigg C, Mogg SL, Osborne R, Dambire C, Boeckx T, Paling Z, Voesenek LACJ, Flashman E, Holdsworth MJ (2018) Oxygen-dependent proteolysis regulates the stability of angiosperm Polycomb Repressive Complex 2 subunit VERNALIZATION2. Nature Communications. 9:5438 DOI:10.1038/s41467-018-07875-7
Gibbs, DJ (2015) Emerging functions for N-terminal protein acetylation in plants. Trends In Plant Science. 20(10): 599-601
Gibbs DJ*, Bacardit J, Bachmair A, Holdsworth MJ* (2014) The eukaryotic N-end rule pathway: conserved mechanisms and diverse functions. Trends in Cell Biology. http://dx.doi.org/10.1016/j.tcb.2014.05.001
Based on your current searches we recommend the following search filters.
Based on your current search criteria we thought you might be interested in these.