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Capillary electrophoresis – mass spectrometry (CE-MS) for protein analysis


Project Description

We are engaged in development of capillary electrophoresis in conjunction with mass spectrometry for bioanalysis. Capillary electrophoresis represents, on one hand, high performance separation technique, which separates molecules in the electric field according to difference in charge and size. Mass spectrometry provides, on the other hand, molecular mass of the analytes thus allowing their identification and structure analysis. The specific separation principle makes capillary electrophoresis interesting alternative to nanoLC-MS for analysis of protein variants or posttranslation modifications such as glycosylation, acetylation or phosphorylation.

A program of this thesis comprise modification of an interface used for transferring analytes from liquid phase in CE to gas phase in MS via nanospray ionization, then optimization of CE separation and nano-ESI and, lastly, data handling by proteomic tools. The candidate should be interested in miniaturised analytical techniques, have sense of precise work and would get familiar with biological background of projects of our collaborators from our institute.

How to apply

To apply for a PhD study at IOCB Prague, you must hold a Master’s degree (MSc) or the equivalent of the MSc in similar field (four or five year undergraduate degree). The application can be submitted before obtaining the Master’s degree, however, the applicant should obtain the degree within five months after the application deadline.

For more information visit our website “Call for PhD applications 2019”:
https://www.uochb.cz/web/structure/797.html

You can apply online via our application form http://www.uochb.cz/phdapplication/index.php/PhDrecruitment

Enquiries

All general enquiries should be directed to Katerina Solcova (). Scientific enquiries can be addressed to the supervisor ().

Funding Notes

Regular monthly income of students at IOCB Prague varies depending on the faculty scholarship and supervisor's financial options.

Related Subjects

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