About the Project
Before they can cause disease, bacteria need to colonise their hosts. To do so they must establish a niche among the resident microbiota. Staphylococcus aureus secretes protein toxins using a Type VII secretion system that kill competitor bacteria. The aim of this project is to characterise a novel family of protein toxins that is secreted by the S. aureus Type VII protein secretion system. The project will involve defining the enzymatic activity and cellular targets of the toxins, and identifying neutralising immunity proteins that protect the host strain from self-intoxication. One or more of the toxins will be purified in complex with their immunity protein for structural analysis. A zebra fish embryo colonisation model will be used to examine the role of the secreted protein toxins and their immunity proteins in interbacterial competition.
This project will provide training in a range of complementary techniques, including microbiology, biochemistry, molecular biology, protein expression and purification, and structural biology.
Informal enquiries may be made to firstname.lastname@example.org
HOW TO APPLY
Applications should be made by emailing email@example.com with a CV and a covering letter, including whatever additional information you feel is pertinent to your application; you may wish to indicate, for example, why you are particularly interested in the selected project/s and at the selected University. Applications not meeting these criteria will be rejected. We will also require electronic copies of your degree certificates and transcripts.
In addition to the CV and covering letter, please email a completed copy of the Newcastle-Liverpool-Durham (NLD) BBSRC DTP Studentship Application Details Form (Word document) to firstname.lastname@example.org, noting the additional details that are required for your application which are listed in this form. A blank copy of this form can be found at: https://www.nld-dtp.org.uk/how-apply.
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