Most of genes in eukaryotes contain introns that are recognised and spliced out by a macromolecular complex, the spliceosome. Spliceosomes are complex and are built of five snRNPs and about 200 proteins. The composition of the spliceosome is not constant and changing during progression from the recognising the intron and building a catalytic core for its removal. Therefore, spliceosomes are very dynamic in re-arranging themselves but these characteristics unlike the transcription complex assembly were not yet fully investigated. The project will address the role of the essential splicing factor PRPF40 in spliceosome assembly. It will focus on isolation and structural characterisation of PRPF40 containing complexes and the kinetics of the transition from the pre-spliceosomal E/A complex to B or fully assembled spliceosome. The isolated complexes will be investigated using electron microscopy and single molecules techniques.
Academic entry requirements: UK Bachelor Degree with at least 2:1 in a relevant subject or overseas equivalent.