Project outline:
A major limit to global food security is annual, preventable yield loss due to plant diseases. Conservative estimates suggest that diseases cause by fungal pathogens are responsible for annual yield losses up to 5-10% globally and widespread fungal disease epidemics are common in our modern monoculture based agricultural system. In order to protect crop plants, farmers rely on a combination chemical control (fungicides) and plant genetic resistance (plant immunity) to prevent disease epidemics. However, these control measures are often short-lived as fungal pathogens rapidly evolve resistance to fungicides or alter their own secreted proteins to evade plant immune recognition. To protect future crop yields it is imperative that we understand how fungal pathogens are able to rapidly evolve in response to chemicals or plant genetic resistance.
The McDonald Lab focuses on high-throughput identification and characterisation of fungal virulence genes using all available genomic technologies. This PhD project will be split into two major themes discussed briefly below:
Theme 1: Genome plasticity and transposon-mediated horizontal gene transfer between fungal wheat pathogens
Our recently published work on the wheat and barley pathogen Bipolaris sorokiniana, a severe wheat disease in Bangladesh, suggests that high rates of chromosomal plasticity exist in naturally infecting populations and that this plasticity contributes to the spread of important virulence genes both within and between species (McDonald, 2019). These pathogenicity genes can be translocated across chromosomes and horizontally transferred between different pathogen species. This project will characterise the extent and frequency of these rapid chromosomal rearrangements in three fungal wheat pathogens. This will involve whole-genome resequencing of a global collection of fungal isolates, followed by comprehensive genome and transposon annotation. We will also characterise chromosomal plasticity using long-read whole genome sequencing coupled with 3D genome re-construction techniques (Hi-C).
Theme 2: Forward genetics screening of genes controlling host-specificity and virulence
This part of the project seeks to understand the molecular basis for host-specificity in the wheat and barley pathogen B. sorokiniana. This fungal pathogen is adept at infecting both of these important crops, causing wide-spread necrosis of leaf tissue. We currently have no understanding of the genes required for infection on either host. To give insight into the genes that control this we will utilise a forward genetics screen to identify mutants that lose the ability to infect either host. Whole-genome re-sequencing of a panel of mutants will be used to identify gene-candidates which will be further investigated using reverse genetics.
Together this PhD will address global food insecurity by identifying the genes responsible for driving horizontal gene transfer and host-specific virulence genes in a devastating fungal pathogen.
https://warwick.ac.uk/fac/cross_fac/mibtp/pgstudy/phd_opportunities/plantandcropscience2023/pathogenicfungi
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