RNA-binding proteins regulate the selection of splice sites in RNA. This is essential for generating productive protein-coding RNA and also for the switching of sites that enables many genes to produce different proteins during processes as diverse as cell differentiation, apoptosis, ageing and memory formation.
We have received major funding (a BBSRC sLOLA award) for a multi-disciplinary investigation of the ways in which RNA-binding proteins control selection. This exciting project aims to transform our understanding of a major step in gene expression, using and developing physical, chemical and biochemical methods to detect protein interactions and complexes. The PhD project will be concerned with pursuing these findings in cells.
The approaches will include knock-down and over-expression of complementary pairs of proteins, mutagenesis to test protein-protein interactions, transcriptome-wide analysis (CLIP-seq) of binding sites of proteins with disrupted interactions, and biotin-proximity ligation to detect interacting partners of proteins in specific complexes in cells.
Academic entry requirements: UK Bachelor Degree with at least 2:1 in a relevant subject or overseas equivalent.