About the Project
This abundant and conserved modification is deposited through a complex called the MLL/SET/COMPASS complex comprising a core complex and Histone MethylTransferase (HMTs) enzymes. Interestingly, lowering the levels of methyl marks at H3K4 using RNAi directed at the core complex increases C. elegans lifespan, but eradicating H3K4 methylation shortens lifespan.
Using ChIP-seq and RNA-seq, we have identified a number of targets that could underscore a new epigenetic programme essential to prevent ageing or promote longevity.
Your project will aim at characterising this epigenetic programme using RNAi, transgenesis, CRISPR, and next generation sequencing technologies such as RNA-seq, ChIP-seq, and ATAC-seq. At the end of your PhD, you will have developed an expertise for both wet lab and bio-informatics using R and Python programming language.
Training/techniques to be provided:
The candidate will have the opportunity to learn a broad range of techniques such as genetics, cell culture, and big data analysis using R and Python as programming language from two laboratories (Poulin and Sharrocks). You will also perform genome editing using CRISPR to engineer new C. elegans strains as well as manipulate the genome of Embryonic Stem Cells. You will have access to core facilities for next generation sequencing as well as state-of-the-art microscopy.
Candidates are expected to hold (or be about to obtain) a minimum upper second class honours degree (or equivalent) in a related area / subject of Biochemistry, Molecular Biology, or Genetics. Candidates with experience in bio-informatics or with an interest in epigenetics and sequencing technology are encouraged to apply.
For international students we also offer a unique 4 year PhD programme that gives you the opportunity to undertake an accredited Teaching Certificate whilst carrying out an independent research project across a range of biological, medical and health sciences. For more information please visit http://www.internationalphd.manchester.ac.uk
As an equal opportunities institution we welcome applicants from all sections of the community regardless of gender, ethnicity, disability, sexual orientation and transgender status. All appointments are made on merit.
Yang SH, Andrabi M, Biss R, Murtuza Baker S, Iqbal M, Sharrocks AD; ZIC3 Controls the Transition from Naive to Primed Pluripotency; Cell Rep. 2019 Jun 11;27(11):3215-3227
Monagan RM, Barnes RG, Fisher K, Andreou T, Rooney N, Poulin GB, Whitmarsh AJ; A nuclear role for the respiratory enzyme CLK-1 in regulating mitochondrial stress responses and longevity; Nat Cell Biol. 2015 Jun;17(6):782-92
Han S, Schroeder EA, Silva-García CG, Hebestreit K, Mair WB, Brunet A. Mono-unsaturated fatty acids link H3K4me3 modifiers to C. elegans lifespan. Nature. 2017 Apr 13;544(7649):185-190
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