About the Project
Aims: We aim to fully characterise the gene expression and protein levels of NF-𝜅B family members (and inhibitors) in pluripotency, the exit from pluripotency, and following differentiation towards the three embryonic lineages. By coupling bulk cell techniques (PCR, Western blotting) and single-cell analyses (flow cytometry, immunofluorescence, state-of-the-art live-cell imaging), we will precisely define the role of NF-𝜅B during these cell-fate transitions, and whether they are modulated by defined NF-𝜅B dynamics.
Training: The IACD is an excellent multidisciplinary research environment with direct links to unique technological platforms and technical expertise, ideal for the student to learn valuable laboratory techniques and skills for their future career. The student will benefit from close interactions with both supervisors, who will be responsible for training them in all aspects of the project, including techniques such as PCR, advanced microscopy, molecular biology, and stem cell culture. Valuable experience will be gained from working within a multidisciplinary environment with regular access to journal clubs, research seminars and group meetings. IACD also runs workshops for students on Experimental Design and Statistical Analysis. Broader research training will be provided through the university’s PGR training programme and doctoral training college. IACD has a strong record in public and patient engagement, and has won awards for past events. Training is available and the student will be strongly encouraged to get involved in and lead such events.
The successful candidate should have, or expect to have, an Honours Degree at 2.1 or above (or equivalent). Candidates whose first language is not English should have an IELTS score of 6.5 or equivalent. This project is most suitable to a student with an undergraduate or Master’s degree in Developmental Biology, Pharmacology, or a related Life Sciences subject (Biology, Genetics, Biochemistry, Biomedical Sciences, Cell Biology, and Medicine).
The Institute of Ageing and Chronic Disease is fully committed to promoting gender equality in all activities. In recruitment we emphasize the supportive nature of the working environment and the flexible family support that the University provides. The Institute holds a silver Athena SWAN award in recognition of on-going commitment to ensuring that the Athena SWAN principles are embedded in its activities and strategic initiatives.
To apply: please send your CV and a covering letter to Dr. David Turner ([Email Address Removed]) with a copy to [Email Address Removed]
Applications accepted all year round.
2. Ashall, L. et al. Pulsatile stimulation determines timing and specificity of NF-kappaB-dependent transcription. Science 324, 242–246 (2009).
3. Turner, D. A. et al. Physiological levels of TNFalpha stimulation induce stochastic dynamics of NF-kappaB responses in single living cells. J. Cell. Sci. 123, 2834–2843 (2010).
4. Tay, S. et al. Single-cell NF-κB dynamics reveal digital activation and analogue information processing. Nature 466, 267–271 (2010).
5. Kim, Y.-E. et al. Upregulation of NF-kappaB upon differentiation of mouse embryonic stem cells. BMB Rep 41, 705–709 (2008).
6. Yang, C. et al. Opposing Putative Roles for Canonical and Noncanonical NFκB Signaling on the Survival, Proliferation, and Differentiation Potential of Human Embryonic Stem Cells. Stem Cells 28, 1970–1980 (2010).
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