Deriving functional neurons from hIPSCs

Neuronal networks grown on petri dishes encode information as electrical activity. This activity is dictated by their underlying structural and functional connectivity. In my lab, we developed a fast and efficient protocol for generating motor neurons from mouse embryonic stem cells. These neurons are positive for the main neuronal markers (b-Tubulin III, MAP2, NeuN) and express markers of excitatory and inhibitory neurotransmitters (Vglut). We want to expand this protocol to human-induced pluripotent stem cells (hIPSCs) and provide evidence that these neurons are functional (i.e. can exhibit action potentials upon stimulation).

The student will learn electrophysiology and will perform patch-clamp and MEA recordings on stem cell derived neurons. This project will take place in the Brain Embodiment Lab within Biomedical Engineering Section of the School of Biological Sciences (SBS), which has a strong reputation for its innovative research in cybernetics, and biomedical engineering, including Brain Computer Interfaces, animats - robots controlled by cultures of living neuronal cells and cognitive robotics systems.

Enquiries:

For informal inquiries please contact Dr. Evangelos Delivopoulos, email: e.delivopoulos@reading.ac.uk

School of Biological Sciences, University of Reading:

The University of Reading, located west of London, England, provides world-class research education programs. The University’s main Whiteknights Campus is set in 130 hectares of beautiful parkland, a 30-minute train ride to central London and 40 minutes from London Heathrow airport.

Our School of Biological Sciences conducts high-impact research, tackling current global challenges faced by society and the planet. Our research ranges from understanding and improving human health and combating disease, through to understanding evolutionary processes and uncovering new ways to protect the natural world. In 2020, we moved into a stunning new ~£60 million Health & Life Sciences building. This state-of-the-art facility is purpose-built for science research and teaching. It houses the Cole Museum of Zoology, a café and social spaces. In the School of Biological Sciences, you will be joining a vibrant community of ~180 PhD students representing ~40 nationalities. Our students publish in high-impact journals, present at international conferences, and organise a range of exciting outreach and public engagement activities.

During your PhD at the University of Reading, you will expand your research knowledge and skills, receiving supervision in one-to-one and small group sessions. You will have access to cutting-edge technology and learn the latest research techniques. We also provide dedicated training in important transferable skills that will support your career aspirations. If English is not your first language, the University's excellent International Study and Language Institute will help you develop your academic English skills.

The University of Reading is a welcoming community for people of all faiths and cultures. We are committed to a healthy work-life balance and will work to ensure that you are supported personally and academically.

Further information:

http://www.reading.ac.uk/biologicalsciences/SchoolofBiologicalSciences/PhD/sbs-phd.aspx