Fluorescent proteins (FPs) have revolutionised microscopy by providing a set of genetically encoded tools that can be used to visualise and study proteins within living systems. Protein chemists have invested considerable effort in fine tuning the properties of these proteins — generating variants that are bright, photostable and span the entire visible spectrum, work which has been recognised with the awarding of two Nobel prizes. An underexploited feature of some FPs, is their propensity for photoswitching; a process in which specific excitation results in a (permanent) change in the emission properties. Harnessing the photochemical control of optical properties provides opportunities to develop novel optically controlled sensors.
In this project you will develop a novel series of FRET – driven photo-switchable fluorescent proteins that will provide a powerful new class of tools can be used for biological imaging and sensing. The project is a collaboration between two labs based in the Department of Chemistry, working with Drs Reid and Craggs. The two labs have considerable expertise in protein chemistry, spectroscopy, and single molecule microscopy, and the project will involve training in all of these areas. The project will involve over expression of target fluorescent proteins, and their covalent modification. These model systems will then be further refined by designing and producing novel interacting protein systems that will be used to demonstrate fine control of the optical properties of our target proteins, both in vitro and in vivo. These proteins will be studied by a variety of biophysical and microscopy techniques with a view to developing a tool kit that can easily be applied in living systems. This interdisciplinary project spans a number of areas in chemistry, biological chemistry, biophysics, and advanced biological imaging, and therefore provides an excellent opportunity for an ambitious student to learn cutting-edge techniques at the interface between the chemistry, physics and the life sciences.
As a PhD student in one of the science departments at the University of Sheffield, you’ll be part of the Science Graduate School. You’ll get access to training opportunities designed to support your career development by helping you gain professional skills that are essential in all areas of science. You’ll be able to learn how to recognise good research and research behaviour, improve your communication abilities and experience the breadth of technologies that are used.
The University of Sheffield is committed to recruiting future scientists regardless of age, ethnicity, gender, gender identity, disability, sexual orientation or career pathway to date. We understand that commitment and excellence can be shown in many ways and we have built our recruitment process to reflect this. We welcome applicants from all backgrounds, particularly those underrepresented in science, who have curiosity, creativity and a drive to learn new skills.
Interested applicants are encouraged to apply via the link provided and list Dr Reid and Dr Cragg's as potential supervisor's. Applicants should have (or expect to obtain) at least the equivalent of a 2.1 honours masters level degree in Chemistry or another appropriate subject. Enquiries should include a CV and cover letter outlining: your academic background, any research experience you have and why you are interested in working on this project.
We expect interviews to take place within one calendar month of the application deadline.
All applications should be made via the online application portal:
https://www.sheffield.ac.uk/postgradapplication/
Full details of what to include in your application can be found at the below web link:
https://www.sheffield.ac.uk/chemistry/postgraduate/phd/apply
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