Coventry University Featured PhD Programmes
FindA University Ltd Featured PhD Programmes
The Francis Crick Institute Featured PhD Programmes
Norwich Research Park Featured PhD Programmes
Cardiff University Featured PhD Programmes

Determining brain glycosylation in ageing and Alzheimer’s disease

About This PhD Project

Project Description

Increasing age is associated with lower levels of cognitive performance; concomitant with this is a decrease in brain glucose metabolism. This decrease is accelerated in Alzheimer’s disease (AD) where reduced brain glucose metabolism is evident prior to the clinical manifestation of dementia suggesting that decreased brain glucose metabolism contributes to the molecular cascade that results in cognitive decline and dementia. We hypothesise that age-dependent alteration in glucose metabolism impact negatively on neuronal function through dysregulation of protein O-GlcNAcylation, a glucose dependent glycosylation. The project will investigate alterations in brain protein O-GlcNAcylation and its regulatory enzymes that occur as we age and which alter as AD progresses using a multidisciplinary approach utilising Western blotting and proteomics methodologies.

Techniques and Methodology
Proteomic analysis, protein purification, mass spectrometry, SDS-PAGE.

Impact on Alzheimer’s research
How brain protein O-GlcNAcylation changes as we age has yet to be determined and there is no consensus about what alterations occur in AD. Protein O-GlcNAcylation is a poorly understood protein post-translational modification although its dysregulation has been identified in a number of other diseases. This research will identify the spectrum of changes in brain protein O-GlcNAcylation that occurs as we age and as AD progresses. It will identify key proteins that distinguish between ageing and Alzheimer’s disease and providing a basis for accelerating new therapeutic agents to combat Alzheimer’s.

Email Now

Insert previous message below for editing? 
You haven’t included a message. Providing a specific message means universities will take your enquiry more seriously and helps them provide the information you need.
Why not add a message here
* required field
Send a copy to me for my own records.

Your enquiry has been emailed successfully

FindAPhD. Copyright 2005-2019
All rights reserved.