This studentship provides world class training in cryo-EM, molecular biology and protein biochemistry. The project will focus on developing cryo-EM methods to characterise proteins to understand the clotting process, a leading cause of morbidity.
Cryo-electron microscopy (cryo-EM) is a powerful tool for determining structures of proteins but the methodology is constantly evolving to improve resolution and the range of possible applications. In Leeds, we have a world leading cryo-EM facility that has access to the some of the best equipment available.
We have isolated Affimers, a protein affinity reagent, against proteins involved in fibrin clotting to investigate conformational changes in fibrinogen during blood clotting and lysis. The project will initially utilize these Affimer reagents to determine their ability to purify these proteins directly from biological samples at a suitable level for cryo-EM. This will be followed by optimisation of sample preparation to determine the best conditions for cryo-EM and then structural determination. Ultimately, this project will provide structural and biochemical understanding of the clotting process.
Funding Note
This studentship is part of the BBSRC White Rose Doctoral Training Partnership in Mechanistic Biology. (https://www.whiterose-mechanisticbiology-dtp.ac.uk/). Appointed candidates will be fully-funded for 4 years. The funding includes:
Tax-free annual UKRI stipend (£15,609 for 2021/22 starts. Awards increase every year, typically with inflation).
UK tuition fees (Around £4,500 per year)
Research Training and Support Grant (RTSG)
Conference and Professional Internships for PhD Students (PIPS) allowances
We aim to support the most outstanding applicants from inside and outside the UK. We are able to offer a limited number of bursaries that will enable full studentships to be awarded to international applicants. These full studentships will only be awarded to exceptional quality candidates, due to the competitive nature of this scheme.
Not all projects will be funded; the DTP will appoint a limited number of candidates via a competitive process.
How to apply
Applicants should complete an online application form and attach the following documentation to support their application.
- a full academic CV
- degree certificate and transcripts of marks
- Evidence that you meet the University's minimum English language requirements (if applicable).
To help us identify that you are applying for this studentship project please ensure you provide the following information on your application form;
- Select PhD in Biological Sciences as your programme of study
- Give the full project title and name the supervisors listed in this advert
- For source of funding please state you are applying for a White Rose BBSRC DTP funded studentship
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