Development and Characterization of a Novel Endothelialized in vitro Model of Human Atherothrombosis

The development of atherothrombosis is a complex and highly regulated process involving the blood vessel wall, platelets, endothelial cells, and plasma proteins. To investigate the mechanisms that lead to atherothrombosis, and develop more effective, antithrombotic treatments, appropriate experimental models are crucial. Currently, thousands of animals are used annually in a variety of in vivo thrombosis models. Standardization of these models between research groups is challenging and results are often variable, requiring large numbers of animals to achieve statistically relevant results. While these studies have significantly increased our understanding of platelet function in a damaged blood vessel, they do not accurately represent human disease. In addition to species differences, current in vivo models lack the endothelial dysfunction and altered blood vessel wall composition associated with cardiovascular disease. It is therefore crucial from both an ethical and scientific perspective that advances are made to reduce animal usage and provide more clinically relevant models in thrombosis research.

The aim of this study is to develop and validate an in vitro model of human atherothrombosis, which reflects the in vivo environment following plaque rupture or erosion and provides an alternative to animal thrombosis models. The model will use human blood, human endothelial cells and haemodynamic forces relevant to those in human coronary arteries to offer a more physiologically relevant platform to investigate human atherothrombosis and test novel antithrombotic treatments.

Applicants should hold (or expect to obtain) a minimum upper-second class honours degree (or equivalent) in biomedical science, biochemistry or related area. Laboratory experience is necessary and knowledge of platelet function or endothelial cell biology would be an advantage, as would a related Masters qualification. Excellent communication skills, strong initiative and good teamwork skills are also crucial.