About the Project
Volatile and non-volatile metabolites have shown to be useful in discriminating bacteria. In this research project, the student will sample headspace and extracellular metabolites from pure cultures to describe a core and resistant metabolite profile. This may also involve isotopic enrichment for metabolic flux analysis. The student will then perform complex univariate and multivariate analyses to develop robust classification algorithms and compare these to commonly used clinical microbiology tests.
Candidate metabolite biomarkers may potentially be used for clinical diagnosis where the student will analyse patient sputa and perform targeted analysis on exhaled breath data from a recent clinical study. The work may lead to developing an assay or screening method for clinical use, which can guide personalised dosing and antimicrobial choice.
Candidates are expected to hold (or be about to obtain) a minimum upper second class honours degree (or equivalent) in a related area / subject. Candidates with experience in microbiology, and analytical chemistry, alongside an interest and aptitude in multivariate data analysis, are encouraged to apply.
For information on how to apply for this project, please visit the Faculty of Biology, Medicine and Health Doctoral Academy website (https://www.bmh.manchester.ac.uk/study/research/apply/). Informal enquiries may be made directly to the primary supervisor.
For international students we also offer a unique 4 year PhD programme that gives you the opportunity to undertake an accredited Teaching Certificate whilst carrying out an independent research project across a range of biological, medical and health sciences. For more information please visit www.internationalphd.manchester.ac.uk
Equality, diversity and inclusion is fundamental to the success of The University of Manchester, and is at the heart of all of our activities. The full Equality, diversity and inclusion statement can be found on the website https://www.bmh.manchester.ac.uk/study/research/apply/equality-diversity-inclusion/
Ahmed WM, Geranios P, White IR, Bromley M, Lawal O, Nijsen T, Goodacre R, Read N, Fowler SJ. Development of an adaptable headspace sampling method for metabolic profiling of the fungal volatome. Analyst 2018 DOI: 10.1039/C8AN00841H
Lawal O, Knobel H, Weda H, Bos LD, Nijsen TME, Goodacre R, Fowler SJ. Volatile organic compound signature from co-culture of lung epithelial cell line with Pseudomonas aeruginosa. Analyst. 2018 Jul 7;143(13):3148-3155. doi: 10.1039/c8an00759d.
Lawal, O., Knobel, H., Weda, H. Nijsen TME, Goodacre R, Fowler SJ. TD/GC–MS analysis of volatile markers emitted from mono- and co-cultures of Enterobacter cloacae and Pseudomonas aeruginosa in artificial sputum Metabolomics 2018 14: 66. doi.org/10.1007/s11306-018-1357-5
Ahmed WM, Lawal O, Nijsen TM, Goodacre R, Fowler SJ. Exhaled Volatile Organic Compounds of Infection: A Systematic Review. ACS Infect Dis. 2017;3:695-710. doi: 10.1021/acsinfecdis.7b00088
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