Development of upconversion nanoparticles for biosensing applications (self funded PhD opportunity)

Key words: nanoparticles, synthesis, lanthanides, biophotonics, biosensing, sustainability

Project Background

Advancement of the biosciences requires new technologies for localized measurements of key molecules within cells and tissues non-invasively. A promising technology for non-invasive biosensing are upconversion nanoparticles (UCNPs), which offer the unique advantageous optical properties of near-infrared excitation and tunable visible emission. These properties arise from a low-phonon energy crystal lattice structure (typically NaYF) containing photonically active lanthanide and transition metal dopants (e.g. Yb, Er, Mn). This enables nano-constructs build around appropriately engineered UCNPs to be detected through thick overlying tissues (on the order of ~1 cm).¹ Such prospects for non-invasive trans-tissue sensing could help improve the sustainability and reduce the ethical burden of biomedical research.

Project Description

Working within a small highly-focused research team, the successful applicant will synthesize UCNPs with varied surface chemistry and photonic properties. These UCNPs will form the core of nano-constructs which can be further modified via inorganic shell formation² and organic molecular loading³ to achieve desired photonic properties and enable a range of potential non-invasive biosensing applications, e.g.  sensing of molecular oxygen, nitric oxide, and pH. UCNP nano-constructs can be loaded with polymers to further enhance biocompatibility and targeting molecules such as antibodies to enable specific targeting. The research program can be tailored to the applicant’s interests, and the end-goal application will be decided on an agile manner, depending on complimentary research in-progress within the group, and the group’s wider strategic aims.

Research Skills Development

The successful applicant will develop a wide range of research skills in nanosensor development and testing. This will include methods such as nanoparticle synthesis, silica shell formation, molecular loading, emission spectroscopy, absorption spectroscopy, scanning/transmission electron microscopy, dynamic light scattering, zeta potential measurement, and Brunauer–Emmett–Teller (BET) analysis. Basic image analysis and data analysis. Full training will be given in these methods. 

The successful applicant will also benefit from undertaking a Postgraduate Certificate in Researcher Professional Development (PGCert-RPD) via the Strathclyde Doctoral School, which will enable the student to gain experience in the themes of knowledge and intellectual abilities; personal effectiveness; governance and organization; engagement, and influence & impact. The successful applicant will also be encouraged to attend local and national academic conferences to present their research, develop their communication skills, and network (pending suitable travel funding). The successful applicant will also develop supervision skills by assisting with supervision of undergraduate students in the laboratory.

Candidate Requirements

Applicants would be expected to hold at least a 2:1 honours degree or equivalent in a relevant subject area (e.g. nanoscience, chemistry, biosciences, photonic materials). Applications from candidates with 2:2 degrees may be considered.