About the Project
The specific objectives of this project:
1. To investigate the likelihood of torpor initiation in commonly used fasting protocols in C57BL/6 mice, such as scheduled feeding used in circadian studies, maintenance of mice on 85% of freely-feeding body weight as used in behavioural studies and overnight fasting as used for glucose tolerance testing.
2. To characterise behavioural performance of animals after torpor. To this end, we shall investigate spontaneous behaviours, use the rotarod task, open field test and novel object recognition task.
3. To define the dynamics of sleep and brain activity which surround a bout of torpor in mice. We will perform electroencephalogram (EEG) recordings during torpor entry, maintenance and arousal out of torpor.
4. To develop the methodology for detecting torpor in food restricted mice. We will use a non-invasive approach, where peripheral body temperature will be monitored in mice undergoing food restriction paradigms.
The project will be co-supervised by Professors Vladyslav Vyazovskiy, Stuart Peirson and David Bannerman (University of Oxford) in collaboration with Dr Sara Wells (MRC Harwell). The project will commence in October 2019 within the Department of Physiology, Anatomy and Genetics at the University of Oxford.
Funding is available for 3 years with a minimum stipend of £14,777 per annum. Informal enquiries may be made to Professor Vladyslav Vyazovskiy at [Email Address Removed]
Interview dates are scheduled for the end of March 2019
2. Hudson, J. W., and Scott, I. M. (1979) Daily torpor in the laboratory mouse, Mus Musculus var. albino. Physilogical Zoology 52, 205-218
3. Gavrilova, O., Leon, L. R., Marcus-Samuels, B., Mason, M. M., Castle, A. L., Refetoff, S., Vinson, C., and Reitman, M. L. (1999) Torpor in mice is induced by both leptin-dependent and -independent mechanisms. Proc Natl Acad Sci U S A 96, 14623-14628
4. Swoap, S. J., Gutilla, M. J., Liles, L. C., Smith, R. O., and Weinshenker, D. (2006) The full expression of fasting-induced torpor requires beta 3-adrenergic receptor signaling. J Neurosci 26, 241-245
5. Schubert, K. A., Boerema, A. S., Vaanholt, L. M., de Boer, S. F., Strijkstra, A. M., and Daan, S. (2010) Daily torpor in mice: high foraging costs trigger energy-saving hypothermia. Biol Lett 6, 132-135
6. Bouma, H. R., WVerhaag, E. M., Otis, J. P., Heldmaier, G., Swoap, S. J., Strijkstra, A. M., Henning, R. H., and Carey, H. V. (2012) Induction of torpor: mimicking natural metabolic suppresion for biomedical applications. J Cell Physiol 227, 1285-1290
7. Vyazovskiy, V. V., Palchykova, S., Achermann, P., Tobler, I., and Deboer, T. (2017) Different Effects of Sleep Deprivation and Torpor on EEG Slow-Wave Characteristics in Djungarian Hamsters. Cereb Cortex 7, 1-12
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