About the Project
Starting from our newest observations we will build this PhD project which will be placed on the interface between cutting edge analysis of bacterial gene regulation and pathogenesis of infection with a contribution of mathematical modelling. The main aim is to nail down the molecular mechanisms driving epigenetic regulation in bacteria. Work will include cloning of synthetic promoter regions with different methylation target sites in both Streptococcus pneumoniae, Lactobacillus casei and Listeria monocytogenes. Quantification of gene expression by reporter luminescence and quantitative PCR will be part of the work. DNA supercoiling and the interactions of histones and transcriptional complexes with synthetic promoter sequences will be tested. Testing the key findings in experimental infection models (4) will aim to translate the pioneering in vitro work to concrete issues characterising treatment and prevention of infection. Findings generated by this project will not only help us to characterise and increase our knowledge of epigenetic-dependent bacterial adaptability mechanisms in environmental and host settings, but also provide new insights into how this recently discovered area of epigenetics can ultimately impact bacterial pathogenesis.
As can be seen from the publication list this work has yielded high-level first-name publications for a number of PhD students (De Ste Croix, Furi, Manso) and this project is aimed to continue in this tradition, by addressing with a unique set of tools a cutting edge question in microbial genomics.
UK/EU applicants only.
Applicants are required to hold/or expect to obtain a UK Bachelor Degree 2:1 or better in a relevant subject.
The University of Leicester English language requirements apply where applicable: https://le.ac.uk/study/research-degrees/entry-reqs/eng-lang-reqs/ielts-65
How to apply:
To apply for the PhD please refer to the guidelines and use the application link at https://le.ac.uk/study/research-degrees/funded-opportunities/bbsrc-mibtp
Please also submit your MIBTP notification form at https://warwick.ac.uk/fac/cross_fac/mibtp/pgstudy/phd_opportunities/application/
Project / Funding Enquiries: [Email Address Removed]
Application enquiries to [Email Address Removed]
UK/EU fees and stipend at UKRI rates. For 2020 this will be £15,285 pa
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