This project will examine the influence of APOE genotype on tau elimination from the brain in traumatic brain injury (TBI) and Alzheimer’s disease (AD). The apoE4 allele is associated with poorer outcome following TBI and is a genetic risk factor for the development of AD, and a prominent pathological feature of both TBI and AD is the accumulation of tau in the brain. Moreover, tau levels are elevated in the brain when apoE4 is expressed compared to other apoE isoforms. Despite the strong pathological presence of tau in TBI and AD and the established correlation between apoE4 and disease progression, there has been little investigation into the relationship between apoE and tau.
The goals of this PhD student project are: 1) determine the interaction between apoE and tau 2) examine the influence of apoE on tau elimination from the brain 3) evaluate the influence of apoE on tau movement in the brain
The techniques associated with this PhD student project are: • Immunoblotting • ELISA • cell culture • isolation of astrocytes • biolayer interferometry • immunohistochemistry • confocal microscopy • delivery of TBI to mice • intracranial administration of molecules in mice • blood and tissue collection from mice