Postgrad LIVE! Study Fairs

Birmingham | Edinburgh | Liverpool | Sheffield | Southampton | Bristol

University of East Anglia Featured PhD Programmes
University College London Featured PhD Programmes
King’s College London Featured PhD Programmes
Imperial College London Featured PhD Programmes
University of Nottingham Featured PhD Programmes

Exploring the ecological and evolutionary impacts of novel agricultural probiotics on naive microbial communities


Project Description

Background
Probiotic inoculants represent a major tool in the shift towards a more sustainable agriculture. The clearest benefits are seen in legumes, like Soya, which form associations with nitrogen fixing bacteria - rhizobia - allowing them to obtain nitrogen from the atmosphere and so reduce the need for carbon intensive fertilizers. Providing crops with effective rhizobia strains can have impressive impacts on yield, especially when crops are being grown outside of their native ranges where their natural rhizobial partners are absent. However, the introduction of high densities of novel bacteria into the soil is likely to have consequences for the local microbial community which are largely unexplored. Novel bacteria may have ecological effects, e.g. washing out of local diversity, or evolutionary effects, e.g. by introducing novel genes to resident bacteria through horizontal gene transfer.

We have the opportunity to study these dynamics at an exciting moment: The UK is on the cusp of the widespread introduction of novel bacteria into a naïve microbial community. At present Soya is emerging as a novel - and sustainable - crop but their natural rhizobial partners (Soya-associated Bradyrhizobium - SABs) are entirely absent from UK soils.

Project description

In this project we will use mesocosm experiments combined with cutting edge sequencing approaches to investigate the ecological and evolutionary effect of SAB introduction on the local microbiome in UK soils. The student will engage with Soya growers across the UK to identify sampling sites, design and conduct greenhouse experiments and develop analytical methods.

The supervisory team brings together an interdisciplinary team formed of experts in rhizobia, soil microbial diversity, bacterial evolution and horizontal gene transfer. In addition, we have the support of our case partner, PlantWorksUK, who specialise in agricultural microbial inoculants. The student will, therefore, be able to acquire a broad set of lab skills (greenhouse experiments, microbiology and molecular biology), analytical techniques (statistics, bioinformatics) and experience in liaising with industry, including the opportunity to undertake a 3 month industrial placement with PlantWorksUK. The project will be primarily based at the university of Sheffield - which is ranked top 5 in the UK for student experience - with opportunities for secondments to work at the James Hutton Institute in Dundee - a world leading institution for crop science.

This project will suit a motivated student interested in using ecology and evolution to support agricultural innovation. The student should have either a 2:1 honours degree + Masters (or equivalent) or first class honours in a relevant subject. This could be Biology, Microbiology, Agricultural Sciences or similar. Experience with either practical microbiology, horticulture or bioinformatics is desirable but not required.

Funding Notes

The studentship joint funded by NERC via the University of Sheffield and the James Hutton Institute/University Joint PhD programme, for a 3.5 year study period. Shortlisted candidates will be interviewed in Jan/Feb 2019. A more detailed plan of the studentship is available to candidates upon application. Funding is available for European applications, but Worldwide applicants who possess suitable self-funding are also invited to apply.

References

Kontopoulou, C.-K., Liasis, E., Iannetta, P.P.M., Tampakaki, A., Savvas, D. (2017) Impact of rhizobial inoculation and reduced N supply on biomass production and biological N2-fixation in common bean (Phaseolus vulgaris L.) grown hydroponically. Journal of the Science of Food and Agriculture 97, 4353-4361

dos Santos, Alves, Silva, Rhem, James & Gross (2017) Diverse genotypes of Bradyrhizobium nodulate herbaceous Chamaecrista (Moench) Fabaceae, Caesalpinioideae) species in Brazil. Systematic and Applied Microbiology, 40, 69-79.

Hall, Brockhurst & Harrison (2017) Sampling the mobile gene pool: innovation via horizontal gene transfer in bacteria, Phil. Trans. R. Soc. B, 372 (1735)

Email Now

Insert previous message below for editing? 
You haven’t included a message. Providing a specific message means universities will take your enquiry more seriously and helps them provide the information you need.
Why not add a message here
* required field
Send a copy to me for my own records.

Your enquiry has been emailed successfully





FindAPhD. Copyright 2005-2018
All rights reserved.