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Exploring the pathogenic roles of Rab GTPases in models of taupathy

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  • Full or part time
    Prof Flaviano Giorgini
    Prof C P Kyriacou
  • Application Deadline
    No more applications being accepted
  • Funded PhD Project (European/UK Students Only)
    Funded PhD Project (European/UK Students Only)

Project Description

Alzheimer’s disease (AD) is the most common neurodegenerative disorder and the predominant cause of dementia. The main pathological hallmarks of the disease in the brain are the presence of amyloid plaques and neurofibrilliary tangles composed of hyperphosphorylated forms of tau (P-tau). While several cellular pathways have been implicated in AD pathogenesis, perturbed membrane dynamics affecting vesicular trafficking, secretion and autophagy likely play a key role in this disease and other neurodegenerative disorders, including Parkinson’s disease (PD) and Huntington’s disease (HD).

The Rab GTPase family of monomeric G proteins plays a critical role in regulating membrane trafficking in cells, with several members being linked to these diseases. Several recent studies have highlighted novel links between specific Rabs and tau biology/pathology. Indeed, phosphorylated RAB10 co-localizes to P-tau in AD patient brains, and both RAB1 and RAB7A have been found to modulate tau secretion in neurons, with Rab7A expression upregulated in specific regions of AD brains. Notably, individuals with mutations in RAB39B exhibit intellectual disability with early-onset PD and related pathology, including tau immunoreactivity. Despite these links, modulation of these and other Rab GTPases has not been conducted in animal models of taupathy in order to clarify their roles in pathogenesis and potential therapeutic value.

We have previously employed the fruit fly Drosophila melanogaster to functionally interrogate Rab GTPases (e.g. Rab11 and Rab8a) in the context of PD and HD. Here we propose to use similar genetic approaches to explore the pathogenic roles of key Rab GTPases in Drosophila and mammalian cell tauopathy models. The student will assess modulation of tau-dependent disease-related phenotypes by candidate Rabs in flies by monitoring viability, lifespan and several behavioural paradigms (e.g. locomotor and circadian activity). Neurodegeneration, synaptic dysfunction and related phenotypes will be studied using biochemical and microscopy approaches in these animals and mammalian cell models. This work will provide important insight into the mechanisms and therapeutic potential of Rab GTPases in AD, and other disorders where tau plays a causative role.

Entry requirements

Applicants are required to hold/or expect to obtain a UK Bachelor Degree 2:1 or better in a relevant subject. The University of Leicester English language requirements apply where applicable.

How to apply

You should submit your application using our online application system (see below).

Apply for a PhD in Genetics

In the funding section of the application please indicate you wish to be considered for a CLS/GGB Studentship

In the proposal section please provide the name of the supervisor and project you want to be considered for.

Funding Notes

Funding Details:
This 3-year studentship is jointly funded by the University of Leicester ‘s College of Life Sciences and the Department of Genetics and Genome Biology. Tuition and bench fees will be covered and a stipend will be paid at the annual Research Council rate (https://www.ukri.org/skills/funding-for-research-training/).

Entry requirements:
Applicants are required to hold/or expect to obtain a UK Bachelor Degree 2:1 or better in a relevant subject. The University of Leicester English language requirements apply where applicable.

References

Breda et al., Hum Mol Genet, 2015, 24(4):1077-91.
Mohamed et al., PLoS One 2017. doi: 10.1371/journal.pone.0178288.
Rodriguez et al., J Neurochem 2018. doi: 10.1111/jnc.13994.
Steinert et al., Hum Mol Genet, 2012, 21(13):2912-22.
Wilson et al., Am J Hum Genet 2014. doi: 10.1016/j.ajhg.2014.10.015.
Yan et al., J Alzheimers Dis 2018. doi: 10.3233/JAD-180023.
Yin et al., Neurobiol Dis, 2014, 70:149-61.
Zafar et al., Mol Neurobiol 2017. doi: 10.1007/s12035-016-9694-8.
Zhang et al., ACS Chem Neurosci 2018. doi: 10.1021/acschemneuro.8b00387.

How good is research at University of Leicester in Psychology, Psychiatry and Neuroscience?

FTE Category A staff submitted: 34.40

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