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Expression systems for pluripotent stem cells and their differentiated derivatives


Project Description

Human pluripotent stem cells provide an opportunity to study normal development, to model disease and to generate cells and products for therapeutic use. A key technology in this system is the ability to over-express genes in a controllable and predictable manner.

We have previously described systems for constitutive expression of transgenes in pluripotent stem cells using specific loci and vector configurations. Although useful for studies involving lineage tracing and cell labelling, these systems do not enable simple manipulation of gene expression in a reliable and controllable manner. This project will create a set of novel vectors for obtaining controllable transgene expression in pluripotent stem cells and their differentiated progeny. Students will learn how to grow, genetically modify and differentiate pluripotent stem cells. The project will also involve techniques such as flow cytometry, immunofluorescence, confocal microscopy, and quantitative PCR. Depending on the direction of the work, generation and analysis of RNAseq data may also be necessary.

The Immune Development Laboratory is physically located at the Murdoch Children’s Research Institute, part of the Melbourne Children’s Campus, which also includes the Royal Children’s Hospital and University of Melbourne: See our website for further information.

https://www.mcri.edu.au/users/professor-ed-stanley.

*To receive a reply to your inquiry, you should provide:
* a 3 sentence paragraph outlining which expression system they would like to explore.
* a copy of your CV
* a copy of your academic transcripts

Funding Notes

Students are enrolled through the Department of Paediatrics, University of Melbourne. Scholarships and Fee Waivers are available from the University on a competitive basis (View Website). Successful students usually require a GPA of 85/100 in order to be competitive. Note that English language requirements apply. Specifically, candidates need to have IELTS score of 7 overall with no individual task score being lower than 7.
* To receive a reply to your inquiry, applicants should provide:
* a 3 sentence paragraph outlining why they interested gene expression systems.
* a copy of their CV
* a copy of their academic transcripts.

References

Publications are most easily viewed by accessing the publicly available google scholar profile of Ed Stanley, or for searching pubmed with the surname of secondary supervisor (elefanty)

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