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FRAP FLIM FAIM – an integrated multimodal fluorescence microscopy technique to study diffusion in cell membranes

  • Full or part time
  • Application Deadline
    Friday, February 14, 2020
  • Funded PhD Project (European/UK Students Only)
    Funded PhD Project (European/UK Students Only)

Project Description

In fluorescence microscopy, obtaining as much information as possible from the fluorophores in the sample in a single experiment is of great interest. We employ Time-Correlated Single Photon Counting (TCSPC) to simultaneously perform Fluorescence Lifetime Imaging (FLIM), Time-Resolved Fluorescence Anisotropy Imaging (tr-FAIM), and Fluorescence Recovery After Photobleaching (FRAP). FLIM yields information about the fluorescence lifetime, which can be sensitive to the microenvironment of the fluorophore. tr-FAIM can monitor the depolarization of the fluorescence, which can be due to rotational motion, or homo-FRET. FRAP shows the translation of the fluorophore. The project will focus on the use of this technique to study rotational and translation diffusion in heterogeneous media such as artificial cell membranes. It is an interdisciplinary project at the boundaries of physics, chemistry and biology.

Subject Areas: Physics, Physical Chemistry, Biology

Start Date: October 2020

Application Procedure

To be considered for the position candidates must apply via King’s Apply online application system at https://apply.kcl.ac.uk/. Details are available at https://www.kcl.ac.uk/physics/postgraduate/research-degrees

Please indicate your desired supervisor and quote research group Biological Physics and Soft Matter in your application and all correspondence.

The selection process will involve a pre-selection on documents, if selected this will be followed by an invitation to an interview. If successful at the interview, an offer will be provided in due time.

Funding Notes

Funding is available for 3.5 years and covers tuition fees at the level set for UK/EU students, c. £5,300 p.a. and a tax-free stipend of approximately £17,000 p.a. with possible inflationary increases after the first year.

References

J.A. Levitt, P.E. Morton G.O. Fruhwirth, G. Santis, P.-H. Chung, M. Parsons and K. Suhling. Simultaneous FRAP, FLIM and FAIM for measurements of protein mobility and interaction in living cells. Biomed Opt Express 6(10) 3842-3854, 2015

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