Chat online with top universities at our virtual study fair - Tuesday 7th July (12pm - 5pm BST)

University of East Anglia Featured PhD Programmes
Xi’an Jiaotong-Liverpool University Featured PhD Programmes
University of Reading Featured PhD Programmes
Applications accepted all year round Self-Funded PhD Students Only

About the Project

A PhD studentship is available to work in the laboratory of Dr. Fiona Wardle (www.kcl.ac.uk/research/profile/wardle-group) in the Randall Centre for Cell and Molecular Biophysics, King’s College London. The successful applicant will be joining a dynamic, interdisciplinary lab, within one of the world’s top research universities

Our laboratory focusses on the networks of gene regulation that control tissue and organ formation in the early vertebrate embryo, and disease progression, particularly cancer, in the adult. We use a combination of approaches to study gene regulation including cell biology, molecular biology, experimental embryology, genomics, and computational biology in a variety of systems including zebrafish, mammalian stem cells and cancer cells.

We are seeking a highly motivated and enthusiastic candidate with a background in developmental biology, biochemistry or computational biology to study gene regulation in cancer metastasis.

Project

This project will investigate the function of enhancer regions in regulating gene expression during cancer progression. Using publicly available genomics data and 4C-seq data generated in house, we have identified putative enhancer regions that regulate the expression of, Brachyury, a transcription factor that promotes metastasis. The candidate will test the activity of these putative enhancers using reporter assays and genome editing (CRISPR), use bioinformatic analysis to identify potential factors that bind the enhancers and test the function of these. The selected candidate will gain experience in cancer cell culture, genomics, molecular biology, microscopy and bioinformatics.

Should candidates be interested in other projects in the laboratory (see: http://www.kcl.ac.uk/research/profile/wardle-group) please feel free to contact Dr Fiona Wardle for an informal chat.

Environment

The candidate will be joining an interdisciplinary team that is based in the Randall Centre at the Guy’s Campus of King’s College London. Our group is part of a vibrant department with numerous researchers using genomics, microscopy, biophysics and computational biology to investigate development and cell biology, and the candidate will gain exposure to numerous techniques and model systems.

The Health Sciences Doctoral Training Centre will also provide methods training and opportunities for career development activities, such as presentation and writing skills (see: kcl.ac.uk/healthsciences).

Eligibility

Prospective candidates should have a 1st or 2:1 level qualification in developmental biology/biochemistry/computational biology or a related programme. Applicants are also required to meet King’s English language requirements (Band D, https://www.kcl.ac.uk/study/postgraduate/apply/entry-requirements/english-language).

To apply, please send a CV, transcript and statement of interest to Dr. Fiona Wardle ().

Funding Notes

There is currently no funding attached to this PhD call and the successful applicant will be expected to provide their own funding. We require candidates that have secured, or wish to secure, their own competitive funding from overseas government agencies or employers. King’s College London provides a list of possible schemes on the following website: View Website.

Note: if you are a prospective student from the African continent, you may be eligible to apply for a Darwin Trust of Edinburgh studentship. Please get in touch with Dr Fiona Wardle for more details.

References

Representative publications from the Wardle group:

Nelson, A.C., Cutty, S.J., Gasiunas, S.N., Deplae, I., Stemple, D.L., Wardle, F.C. (2017). In Vivo Regulation of the Zebrafish Endoderm Progenitor Niche by T-Box Transcription Factors. Cell Reports 19(13):2782-2795.

Windner, S.E., Doris, R.A., Ferguson, C.M., Nelson, A.C., Valentin, G., Tan, H., Oates, A.C, Wardle, F.C., Devoto, S.H. (2015). Tbx6, Mesp-b and Ripply1 regulate the onset of skeletal myogenesis in zebrafish. Development, 142(6):1159-68.

Nelson, A.C., Cutty, S.J., Niini, M., Stemple, D.L., Flicek, P., Houart, C., Bruce, A.E.E and Wardle, F.C. (2014). Global identification of Smad2 and Eomesodermin targets in zebrafish identifies a conserved transcriptional network in mesendoderm and a novel role for Eomesodermin in repression of ectodermal gene expression. BMC Biology, 12(1):81.

Nelson, A.C., Pillay, N., Henderson, S., Presneau, N., Tirabosco, R., Halai, D., Berisha, F., Flicek, P., Stemple, D.L., Stern, C., Wardle, F.C., Flanagan, A.M. (2012). A gene regulatory network directed by T, brachyury gives insight into the pathogenesis of chordoma. Journal of Pathology, 228, 274-85.

Jahangiri, L., Nelson, A.C., Wardle, F.C. (2012). A cis-regulatory module upstream of deltaC regulated by Ntla and Tbx16 drives expression in the tailbud, presomitic mesoderm and somites. Developmental Biology, 37, 110-20.

Garnett, A.T., Han, T.M., Gilchrist, M.J., Smith, J.C., Eisen, M.B., Wardle, F.C., Amacher, S.L. (2009). Identification of multiple direct T-box target genes in the developing zebrafish mesoderm. Development 136, 749-60.

Morley, R.H., Lachani, K., Keefe, D., Flicek, P., Gilchrist, M.J., Smith, J.C., Wardle, F.C. (2009). A gene regulatory network directed by zebrafish No tail accounts for its roles in mesoderm formation. Proc Natl Acad Sci U S A 106, 3829-34.

Email Now

Insert previous message below for editing? 
You haven’t included a message. Providing a specific message means universities will take your enquiry more seriously and helps them provide the information you need.
Why not add a message here

The information you submit to King’s College London will only be used by them or their data partners to deal with your enquiry, according to their privacy notice. For more information on how we use and store your data, please read our privacy statement.

* required field

Your enquiry has been emailed successfully





FindAPhD. Copyright 2005-2020
All rights reserved.