About the Project
Nevertheless, there remain significant gaps in our knowledge about how the Sec machinery functions in living cells. For example, an array of auxiliary Sec components (e.g. SecD, SecF, YajC, YidC) associate with SecYEG in vivo and assist translocation. However, it is not clear what these proteins are doing. Furthermore, recent work by our group indicates that there is at least one previously undiscovered accessory Sec component (which we have named AscA) that assists translocation, and there could be more. Understanding the functions and molecular mechanisms of these components will be critical to understanding this fundamental biological process. In addition, insight into the mechanism of protein translocation in vivo would allow us to engineer cells that more efficiently produce secreted proteins and could lead to advances in protein production for biotechnology.
The goal of this project will be to determine the functions of these auxiliary and accessory Sec components in vivo. The student will use high-throughput genetic screening methods to discover these functions and potentially to discover new Sec accessory components. In addition, the student will cutting-edge methodologies in molecular genetics and biochemistry to investigate the molecular mechanism of these components in vivo.
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