About the Project
In this project with C. elegans, the goal is to establish the role of the diet and the gut microbiome in healthy aging. C. eleganshas close association with bacteria, which are both food source and part of the gut microbiome. In our preliminary experiments using the bacterium Bacillus subtilis, we found mutants that can increase C. eleganslifespan and healthspan by 50%.
The goal of the project is to determine the metabolites produced by the bacteria that influenceC. elegans lifespan, as well as the genetic pathways in C. elegansthat are influenced by the bacteria.
[Techniques that will be undertaken during the project:
- For the characterization of the metabolites produced by mutant bacteria, we will use metabolomics and proteomics approaches.
- For understanding the role of bacteria on gene expression in the nematode, we will use transcriptomics approaches as well as transgenic nematodes with reporter genes, or generate mutants.
- To essay for lifespan, we will use microfluidic devices to determine health span.
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