This fully funded, 4-year PhD project is part of a competition funded by the BBSRC EASTBIO Doctoral Training Partnership.
Project Overview
How do sexually reproducing organisms maintain genome integrity while amplifying diversity? This project aims to unravel the fundamental principles behind building robust cellular systems. By using budding yeast meiosis as a model, we delve into the mechanisms that safeguard the genome during the formation of haploid gametes, such as eggs and sperm, from diploid cells. Through genetic editing and multi-omics analysis, we seek to unlock the secrets of genome reshuffling, diversity generation, and the prevention of chromosome number aberrations, which can lead to conditions like Down's syndrome.
Addressing the Core Problem
Meiosis is a complex process involving DNA replication and chromosome segregation, pivotal for creating genetic diversity. Central to this process is the formation and repair of DNA double-strand breaks (DSBs) through homologous recombination. Failure to recombine chromosomes can result in severe disorders. This project aims to dissect the molecular mechanisms regulating meiotic recombination, shedding light on the principles that underpin the robustness of sexual reproduction.
Approach
You will investigate the relationship between DNA replication timing and recombination. Specifically, you will examine whether early-replicating domains preferentially recombine and how modifying replication timing affects recombination patterns. You will also explore the consequences of recombination without prior replication. Using budding yeast as a model system, we have the tools and expertise to answer these questions. Insights from the project hold the potential to benefit not only cellular systems but also reproductive medicine.
Skills You Will Acquire
As a member of our team, you will gain proficiency in both wet and dry laboratory skills. You will collect data using a range of techniques in Molecular Genetics/Genomics/Proteomics, including genome editing, chromatin immunoprecipitation, immunostaining, ChIP-seq, and mass spectrometry, and analyse them by bioinformatics tools like R. Our national and international collaborations provides access to state-of-the-art technologies and a diverse network. This program includes a mandatory three-month off-campus internship, enriching your skill set and networking opportunities.
Environment
Located within the Institute of Medical Sciences (IMS) at the University of Aberdeen, our lab thrives on interdisciplinary collaboration. Dr Murakami, the main supervisor, brings expertise in meiosis research and will closely guide your work. You will also benefit from the knowledge of Dr Lorenz and Professor Donaldson, specialists in meiosis and DNA replication. Join our vibrant community of cellular and molecular biology labs, participate in weekly joint meetings, and immerse yourself in an environment that fosters growth and innovation.
---------------------------------
ELIGIBILITY:
Applicants should hold a minimum of a 2:1 UK Honours degree (or international equivalent) in a relevant subject. Those with a 2:2 UK Honours degree (or international equivalent) may be considered, provided they have (or are expected to achieve) a Distinction or Commendation at master’s level.
All students must meet the eligibility criteria as outlined in the UKRI guidance on funding for postgraduate training and development. This guidance should be read in conjunction with the UKRI Training Grant Terms and Conditions.
---------------------------------
APPLICATION PROCEUDRE:
- Please visit this page for full application information: How to apply | eastbio (eastscotbiodtp.ac.uk)
- Please send your completed EASTBIO application form, along with academic transcripts to Alison Innes at [Email Address Removed]
- Two references should be provided by the deadline using the EASTBIO reference form. References should be sent to [Email Address Removed]
- Unfortunately, due to workload constraints, we cannot consider incomplete applications.
- CV's submitted directly through a FindAPhD enquiry WILL NOT be considered.
- If you require any additional assistance in submitting your application or have any queries about the application process, please don't hesitate to contact us at [Email Address Removed]
Continue with Facebook
