About the Project
Our bones have many essential roles in our lives, including weight-bearing, ion homeostasis and allowing locomotion by anchoring the muscles. During childhood they must fulfill these functions as well as one more: they must grow so that we reach our adult height. The PhD student will study how the long-bones grow and aim to understand what maintains this growth throughout childhood.
The successful candidate will plan and conduct experimental research to reveal fundamental mechanisms of human growth. Duties will include collecting human and mouse growth plate samples and using molecular biology techniques to analyse them. Methodologies include sequencing experiments at the genomic and transcriptomic levels (RNAseq and spatial transcriptomics), confocal microscopy, laser capture micro-dissection, histological and histochemical staining, immunofluoresence, in situ hybridisation and other molecular biology technologies.The student will learn how to conduct bioinformatic analyses of their generated data.
The successful applicant will acquire basic and specialized knowledge and understanding of skeletal growth, stem cell renewal, and computational analyses.
Full details and a link to the application portal can be found using the following link: https://kidoktorand.varbi.com/en/what:job/jobID:376902/.
A radical switch in clonality reveals a stem cell niche in the epiphyseal growth plate
Newton PT, Li L, Zhou B, Schweingruber C, Hovorakova M, Xie M, Sun X, Sandhow L, Artemov AV, Ivashkin E, Suter S, Dyachuk V, El Shahawy M, Gritli-Linde A, Bouderlique T, Petersen J, Mollbrink A, Lundeberg J, Enikolopov G, Qian H, Fried K, Kasper M, Hedlund E, Adameyko I, Sävendahl L, Chagin AS;
Nature 567(7747):234-238 (2019)
Postnatal skeletal growth is driven by the epiphyseal stem cell niche: potential implications to paediatrics (Review)
Chagin AS, Newton PT;
Pediatric Research. 87(6):986-990 (2019)
Clonal Genetic Tracing using the Confetti Mouse to Study Mineralized Tissues
Zhou B, Kaucka M, Chagin AS, Newton PT;
J. Vis. Exp. (152), e60424 (2019)
Superficial cells are self-renewing chondrocyte progenitors, which form the articular cartilage in juvenile mice
Li L, Newton PT, Bouderlique T, Sejnohova M, Zikmund T, Kozhemyakina E, Xie M, Krivanek J, Kaiser J, Qian H, Dyachuk V, Lassar AB, Warman ML, Barenius B, Adameyko I, Chagin AS;
FASEB 31(3):1067-1084 (2016)
Pharmacological inhibition of lysosomes activates the mTORC1 signaling pathway in chondrocytes in an autophagy independent manner
Newton PT, Vuppalapati KK, Bouderlique T, Chagin AS;
Autophagy 11(9):1594-607 (2015)
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