About the Project
About PhD project
Successful pathogens secrete virulence proteins (effectors) during plant colonisation. Effectors are highly diverse, often uncharacterised proteins that function to suppress immunity or disrupt plant cellular function. Our previous work has revealed that there is a highly diverse effector repertoire in the fungus Z. tritici (Kettles et al. 2017, Kettles et al. 2018). Similar to other pathogens, many Z. tritici effectors are highly polymorphic with few putative domains or functions. We have also identified effectors with similarity to broad-spectrum toxins that may be involved in interaction with host plants or other microorganisms in the environment. Despite this work, it is still unknown how most effectors contribute to disease or are differentially recognised during infection of either host (wheat) or non-host plants. This project will investigate the extent to which effector recognition contributes to disease resistance against this pathogen.
This project aims to address three key areas:
(1) to determine the functions of Z. tritici effectors during interactions with both host and non-host plants,
(2) to assess effector diversity and subsequent impact on function across global Z. tritici populations, and
(3) to identify effector recognition and immune signalling components in plants.
This project will feature both wet-lab and computational components. The successful applicant will gain extensive experience of functional genomics, molecular biology, biochemistry, fungal handling and pathoassays, and techniques associated with protein expression and purification. There will be opportunity to perform computational genomic analysis using a global Z. tritici isolate collection to assess effector diversity. The project will also use transcriptomic analysis (RNAseq) to assess plant responses to effector variants.
For any informal enquiries, please contact [Email Address Removed]. Further details on the Kettles lab can he found here (https://www.birmingham.ac.uk/staff/profiles/biosciences/kettles-graeme.aspx). Details on how to apply for this project can be found on the MIBTP website (https://warwick.ac.uk/fac/cross_fac/mibtp/pgstudy/phd_opportunities/plantandcrop2020/combat)
MIBTP is a BBSRC funded Doctoral Training Partnership between the universities of Aston, Birmingham, Harper Adams, Leicester and Warwick. Students from a wide diversity of academic backgrounds are encouraged to apply: those with creative drive in both theoretical disciplines (for example, maths, computer science, statistics) as well as experimental science (for example, biology, biomedicine, chemistry, biotechnology). Studentships include: fees (cost of UK fee rate), a tax free annual stipend, a travel allowance in year 1, a travel / conference budget, a generous consumables budget and use of a MacBook Pro for the duration of the programme.
Kettles et al. New Phytol. 2018 217(1):320-331.
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