About the Project
Many of the regulatory roles of ISG15 occur inside the cell as it is involved in controlling IFN-I signalling. However, ISG15 is also secreted and functions as a cytokine. The most compelling evidence for this is that ISG15 loss-of-function is one of the genetic causes of Mendelian susceptibility to mycobacterial disease (MSMD), a rare condition associated with predisposition to weakly virulent mycobacteria, including the BCG vaccine. Patients with MSMD are unable to produce a cytokine known as IFN-gamma (IFN-γ) from NK cells and so are unable to mount a proper T cell response to infection. It was later found that secreted ISG15 is required for NK cells to produce IFN-γ. It is also known that secreted ISG15 regulates the expression of several other factors implicated in the immune response (such the chemokine CXCL10); but the molecular details of this are completely unknown.
Based on the hypothesis that ISG15 is a critical link between innate immunity (IFN-I) and the adaptive immune response, this project will address some of the fundamental questions related to how secreted ISG15 shapes our immune response to infection.
This highly focussed project, which has implications for our fundamental understanding of host defence, health and disease, is designed to provide training in molecular virology, recombinant protein purification and characterisation, CRISPR/Cas9 genome editing technologies, cutting-edge proteomics and FACS analyses.
Infection and immunity is clearly a subject considered a priority area of research across the world. The skills developed during this studentship will place the successful candidate in a strong position to pursue a career in this area (which may include employment in healthcare, the private sector or to continue in academia). Students at St Andrews University have access to award-winning training that recognises the value of an integrated workforce as well as providing personal development programmes that complement their laboratory training.
You will be part of a productive and supportive research team that encourages personal development by providing training opportunities beyond the lab.
Please direct informal enquiries to Dr David Hughes ([Email Address Removed])
Applications can be made online via our online portal- https://www.st-andrews.ac.uk/study/apply/postgraduate/research/
Based on your current searches we recommend the following search filters.
Based on your current search criteria we thought you might be interested in these.