How does the TRAIP ubiquitin ligase guard the mammalian genome from damage?

The TRAIP ubiquitin ligase is a key regulator of genome integrity in mammalian cells. The importance of TRAIP is highlighted by the fact that mutation leads to a human disease syndrome, whilst over-expression of TRAIP can be associated with human cancer. Recent work has indicated that TRAIP associates with the replisome and ubiquitylates potential barriers in front of DNA replication forks, to activate DNA repair pathways. In addition, TRAIP is regulated during mitosis to destroy any remaining replisomes and allow for rapid processing of replication forks to facilitate chromosome segregation. It is likely that TRAIP has additional important targets that remain to be identified. This project will use a combination of cutting edge biochemical and molecular genetic techniques to identify and characterise novel targets of TRAIP, to explore their role in preserving genome integrity in mammalian cells. As well as being an exciting project in a fascinating area of fundamental cell biology, such new targets of TRAIP are also likely to be important in the context of human disease.

At the MRC PPU, as well as the possibility of a PhD in one particular lab, we offer the possibility of two 4.5-month rotations in labs of their choice. A range of other projects from MRC PPU scientists are advertised on this website. Rotations provide valuable experience and help with deciding on the choice of PhD project and research group.

How to Apply

Please send a CV with contact details of three referees to and a cover letter explaining why you have chosen to apply to MRC PPU to [Email Address Removed]. The closing date for applications is 14th April 2023. Applications from overseas students are welcome.