About the Project
The aim of this project is to understand how key regulatory immune pathways rewire CD4+ T cell metabolism to suppress effector characteristics of T cells, dampening tissue-damaging inflammation. To address this aim, highly tractable in vitro assays will be used alongside complex in vivo models of inflammation, including malaria and helminth infections. The project will utilise cutting edge combinations of intravital two photon microscopy, transcriptomics, metabolic flux analyses and flow cytometry. Combined, these approaches will delineate the spectrum of action of key regulatory cytokines on effector T cell function, and will show how specific perturbation of metabolic events dominantly modifies T cell activity.
Training in immunology, microscopy, flow cytometry and systems biology will be provided to fit student’s background and interests. The student will join a team of interdisciplinary researchers who have established all of the experimental techniques required for the project. Dr Couper’s background is in malaria immunology, with a focus on in vivo and in vitro analysis of T cell function. Professor Grencis’s background is in helminth immunology, specifically how Th2 responses develop and are regulated. The core facilities at the University of Manchester will provide significant expertise in imaging, genomic technologies, and bioinfomatics.
The student will use and receive training in microscopy (two-photon and confocal), metabolic flux analyses (Seahorse analyser), transcriptomics and a variety of cutting edge immunological techniques, such as multiparameter flow cytometry. The student will also obtain training in in vivo experimentation.
This project has a Band 3 fee. Details of our different fee bands can be found on our website (View Website). For information on how to apply for this project, please visit the Faculty of Biology, Medicine and Health Doctoral Academy website (View Website).
Informal enquiries may be made directly to the primary supervisor.
2. T cell metabolism drives immunity. Buck MD, O'Sullivan D, Pearce EL. J Exp Med. 2015 Aug 24;212(9):1345-60. doi: 10.1084/jem.20151159.
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